食品科学 ›› 2007, Vol. 28 ›› Issue (8): 285-289.

• 生物工程 • 上一篇    下一篇

基因工程菌E.coli BL21/pET-DsbA-MalQ发酵产麦芽糖转糖基酶的研究

 王水兴, 李燕萍, 许杨, 夏慧玲, 吴凌伟, 郁振军   

  1. 南昌大学中德联合研究院; 南昌大学中德联合研究院 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Study on Fermentation of Recombinant Strain E.coli BL21 /pET-DsbA-MalQ to Produce Amylomaltase

 WANG  Shui-Xing, LI  Yan-Ping, XU  Yang, XIA  Hui-Ling, WU  Ling-Wei, YU  Zhen-Jun   

  1. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 为了获得最佳的基因工程菌E.coli BL21/pET-DsbA-MalQ发酵产酶条件,通过进行诱导时间﹑诱导温度﹑IPTG的浓度﹑诱导前菌液浓度(OD600)等因素梯度实验,研究各因素对发酵产酶的影响。实验结果表明,基因工程菌的最佳诱导条件分别为:诱导时间为2.5h,诱导温度为30℃,IPTG浓度为0.5mmol/L,诱导前OD600值为0.5。在最佳工艺条件下,粗酶液的酶活为130U,相当于出发菌产酶效率的135倍。

关键词: E.coli BL21/pET-DsbA-MalQ, 诱导时间, 诱导温度, IPTG浓度, 麦芽糖转糖基酶

Abstract: The effect of inducing time, inducing temperature, concentration of IPTG and concentration of cells (OD600) on fermentation of E.coli BL21/pET-DsbA-MalQ were studied. The optimum conditions were inducing time 2.5 h, inducing temperature 30 ℃, concentration of IPTG 0.5 mmol/L and concentration of cells (OD600) 0.5 respectively. When the E.coli BL21/pET-DsbA-MalQ was incubated at the optimum conditions, the activity of crude enzyme was 130 U, and it was 135 times of enzyme activity of initial strain.

Key words:  , E.coli BL21/pET-DsbA-MalQ; inducing time; inducing temperature; concentration of IPTG; amylomaltase;