食品科学 ›› 2007, Vol. 28 ›› Issue (8): 303-306.

• 生物工程 • 上一篇    下一篇

黄伞胞外多糖液体培养基的优选

 卜庆梅, 韩立亚, 黄清荣, 杨圣勇   

  1. 鲁东大学生命科学学院; 鲁东大学生命科学学院 山东烟台264025; 山东烟台264025;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Optimization of Liquid Medium for Exopolysaccharide Production of Pholiota adiposa

 BU  Qing-Mei, HAN  Li-Ya, HUANG  Qing-Rong, YANG  Sheng-Yong   

  1. College of Life Science, Ludong University, Yantai 264025, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 采用液体培养和正交试验方法研究了不同碳、氮源及不同无机盐浓度对黄伞产生胞外多糖的影响。结果表明,适宜黄伞产生胞外多糖的碳源为蔗糖、麦芽糖、红糖,适宜氮源为麦麸、酵母粉、NH4NO3;通过正交试验确定了产胞外多糖的最优液体培养基为:蔗糖2%、麦麸3%、MgSO40.15%、KH2PO40.25%。

关键词: 黄伞, 胞外多糖, 正交试验, 碳源, 氮源

Abstract: The effect of carbon sources, nitrogen sources and different concentration of inorganic salt on the production of exopolysaccharide of Pholiota adiposa was studied in this paper by liquid culturing and orthogonal test. The results showed that the suitable carbon sources for exopolysaccharide produced by Pholiota adiposa were sucrose, maltose and brown sugar and the suitable nitrogen sources were wheat bran, yeast extract power and NH4NO3. The best liquid medium proved by the orthogonal experiment was sucrose 2%, wheat bran 3%, MgSO4 0.15% and KH2PO4 0.25%.

Key words: Pholiota adipose, exopolysaccharide, orthogonal test, carbon sources, nitrogen sources