饲料中的转基因成分在家禽体内代谢残留的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (8): 428-432.

饲料中的转基因成分在家禽体内代谢残留的研究

王长康, 陈文炳, 邵碧英, 江树勋, 杨婕, 李寿崧, 郭立新

福建农林大学动物科学学院；福建出入境检验检疫局；福建出入境检验检疫局福建福州350002；福建福州350001；

出版日期:2007-08-15 发布日期:2011-10-24

PCR Analysis on Metabolic Remains of Transgenic Component Derived from Feed in Poultry Bogy

WANG Chang-Kang, CHEN Wen-Bing, SHAO Bi-Ying, JIANG Shu-Xun, YANG Jie, LI Shou-Song, GUO Li-Xin

1.College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001, China

Online:2007-08-15 Published:2011-10-24

摘要/Abstract

摘要： 用含20%抗草甘膦转基因豆粕的饲料喂养蛋鸡、番鸭,通过检测其内脏、肌肉、血液、粪便及肠道微生物的豆粕转基因成分,探讨饲料中转基因成分在家禽体内的代谢残留状况。以定性PCR检测含20%转基因豆粕的饲料及蛋鸡、番鸭的八种内脏样品(肠、胰腺、食道、肝、腺胃、肾、心脏、肌胃)、肌肉、血液、蛋品、粪便、肠道微生物的转基因豆粕的工具基因CaMV35S启动子与NOS终止子以及目的基因CP4-EPSPS等外源基因成分。PCR检测结果表明:除了含20%转基因豆粕的饲料外,蛋鸡、番鸭的八种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未检出转基因豆粕的CaMV35S、NOS、CP4-EPSPS等外源基因成分。检测结果表明:长期喂养含20%转基因豆粕饲料的蛋鸡、番鸭的八种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未发现有转基因成分残留,说明饲料中的转基因成分,通过肠胃消化后充分降解,不会在鸡、鸭体内残留,也不会通过粪便排泄而扩散到土壤环境中。

关键词: 转基因饲料, 转基因成分, 家禽, 代谢残留

Abstract: The present study aimed to probe into the metabolic remains of transgenic components derived from feed in the 8 inner organs including intestines, pancreas, esophagus, liver, preventriculus, kidney, heart, gizzard as well as muscle, blood, egg, dejecta, intestinal bacteria of laying hens and muscovy ducks fed with feed containing 20% genetically modified soybean cake. The laying hens and muscovy ducks fed by feed containing 20% genetically modified soybean cake were used as test materials. The transgenic components including CaMV35S, NOS, CP4-EPSPS derived from feed were analyzed by the qualitification PCR. These transgenic components were found only in feed containing 20% genetically modified soybean cake, but were not found in the 8 inner organs, muscle, blood, egg, dejecta, intestinal bacteria of laying hens and muscovy ducks tested. The results indicated that the metabolic remains of transgenic components from genetically modified soybean cake in feed were not found because of being digested fully. These transgenic components also don’t spread to environment through dejecta excretion. It means that the genetically modified soybean cake is safe to poultry products, human and environment.

Key words: genetically modified feed, transgenic component, poultry, metabolic remains

王长康, 陈文炳, 邵碧英, 江树勋, 杨婕, 李寿崧, 郭立新. 饲料中的转基因成分在家禽体内代谢残留的研究[J]. 食品科学, 2007, 28(8): 428-432.

WANG Chang-Kang, CHEN Wen-Bing, SHAO Bi-Ying, JIANG Shu-Xun, YANG Jie, LI Shou-Song, GUO Li-Xin. PCR Analysis on Metabolic Remains of Transgenic Component Derived from Feed in Poultry Bogy[J]. FOOD SCIENCE, 2007, 28(8): 428-432.

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[5]	陈彦1,2，潘见1,*，王亚2，惠爱玲1，杨毅1. 大豆DNA提取方法及降落PCR检测转基因成分的研究[J]. 食品科学, 2009, 30(24): 355-358.
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[7]	孙红炜, 路兴波, 杨崇良, 尚佑芬, 赵玖华, 王升吉. 9种大豆制品中转基因成分定性PCR检测[J]. 食品科学, 2008, 29(2): 234-237.
[8]	刘宗德，马霞. 法国家禽质量认证系统对我国食品质量和安全保障体系的启示[J]. 食品科学, 2006, 27(12): 847-850.
[9]	邵碧英, 陈文炳, 杨婕. 马铃薯及其制品中转基因成分的多重PCR检测[J]. 食品科学, 2006, 27(1): 178-181.
[10]	邵碧英, 陈文炳. 玉米及其制品中转基因成分的单一PCR及多重PCR检测[J]. 食品科学, 2005, 26(9): 380-384.
[11]	邵碧英, 江树勋, 陈文炳, 李寿崧. 番茄、甜椒中转基因成分和内源基因的多重PCR检测方法的建立[J]. 食品科学, 2004, 25(10): 219-223 .
[12]	陈文炳, 江树勋, 邵碧英, 李寿崧, 朱晓南, 王泽生, 廖建华. 常见食用菌中转基因成分定性PCR检测方法的建立[J]. 食品科学, 2004, 25(10): 206-210.
[13]	徐宝梁, 王炳武, 吴亚君, 陈颖, 苏宁, 白双义, 张青文. 大豆异黄酮中转基因大豆成分检测研究[J]. 食品科学, 2004, 25(1): 127-130.
[14]	陈坤杰, 刘德营. 关于传统中式禽肉制品的工业化生产[J]. 食品科学, 2003, 24(3): 150-153.
[15]	汪丰. 注射过激素的肉鸡特征及外观鉴别[J]. 食品科学, 1989, 10(7): 51-53.