酒精和高脂复合型小鼠肝损伤模型的建立

食品科学 ›› 2007, Vol. 28 ›› Issue (8): 478-480.

酒精和高脂复合型小鼠肝损伤模型的建立

王政, 张静, 惠伯棣

北京联合大学应用文理学院生物学系；北京联合大学应用文理学院生物学系北京100083；北京100083；

出版日期:2007-08-15 发布日期:2011-10-24

Establishment of Alcohol and Fat-induced Liver Injury Mode in Mouse

WANG Zheng, ZHANG Jing, HUI Bo-Di

Department of Biology, College of Applied Arts and Science, Beijing Union University, Beijing 100083, China

Online:2007-08-15 Published:2011-10-24

摘要/Abstract

摘要： 目的:建立脂肪堆积和慢性酒精性损伤复合型的小鼠肝损伤模型。方法:小鼠被随机分为模型组和对照组。模型组每天灌胃酒精,同时给予高脂饲料,对照组每天灌胃等量的水,给予正常饲料。灌胃4周后,采集被测试动物的肝脏,检测肝匀浆液的丙二醛(MDA)和谷胱甘肽(GSH)2项指标;采集被测试动物的血,检测谷丙转氨酶(ALT)、血清天门冬氨酸氨基转移酶(AST)和血清乳酸脱氢酶(LDH)3项指标。结果:灌胃4周后,模型动物组谷丙转氨酶(ALT)、血清天门冬氨酸氨基转移酶(AST)、血清乳酸脱氢酶(LDH)活力均显著增高,肝脏MDA指标升高(p<0.05),GSH显著降低(p<0.05)。结论:在灌胃酒精的同时饲喂小鼠高脂饲料,可以成功制备脂肪积累和慢性酒精损伤复合型小鼠肝损伤模型。

关键词: 肝损伤模型, 小鼠

Abstract: Objective: Establish of alcohol and fat-induced liver injury mode in mouse. Methods: Selected mice were randomly divided into two groups, one was trial group and the other was control group. Optimized portions of alcohol and high-fat feed were administrated gastrically every day for trail group while the same volumes of water and normal feed were applied for control group. After 4 weeks, livers and blood were collected from animals for 2 (MDA and GSH) and 3 (ALT, AST and LDH) assessments, respectively. Results: A significant increase in ALT, AST, LDH and MDA was observed while a reduction of GSH was seen. Conclusion: A supply of alcohol and high-fat feed by gastric administration is able to establish liver injury mode in mouse.

Key words: liver injury mode, mouse

王政, 张静, 惠伯棣. 酒精和高脂复合型小鼠肝损伤模型的建立[J]. 食品科学, 2007, 28(8): 478-480.

WANG Zheng, ZHANG Jing, HUI Bo-Di. Establishment of Alcohol and Fat-induced Liver Injury Mode in Mouse[J]. FOOD SCIENCE, 2007, 28(8): 478-480.

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