食品科学 ›› 2007, Vol. 28 ›› Issue (8): 49-55.

• 基础研究 • 上一篇    下一篇

海藻糖和透明质酸对膜脂双层的保护及其作用机制

 张玉华, 籍保平, 凌沛学, 孟一   

  1. 山东师范大学生命科学学院; 中国农业大学食品科学与营养工程学院; 山东省生物药物研究院博士后科研工作站; 山东商业职业技术学院 山东济南250014中国农业大学食品科学与营养工程学院; 北京100083; 山东济南250108; 山东济南250013;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Protective Effects and Mechanisms of Trehalose and Hyaluronic Acid on Membrane Lipid Bilayer

 ZHANG  Yu-Hua, JI  Bao-Ping, LING  Pei-Xue, MENG  Yi   

  1. 1.College of Life Science, Shandong Normal University, Jinan 250014, China; 2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 3.Working Station for Postdoctoral Scientific Research, Institute of Biopharmaceuticals of Shandong Province, Jinan 250108, China;4.Shandong Institute of Commerce and Technology, Jinan 250013, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 根据冻干脂质体药物保存率、粒径变化、玻璃化温度(Tg)以及磷脂与糖类相互作用,考察海藻糖和透明质酸(HA)的保护作用及其作用机制。冻干-再水化过程中,海藻糖、乳糖、蔗糖、HA以及海藻糖和HA复配物均能抑制脂质双层融合与药物泄漏。HA单独使用时保护作用较低,而与海藻糖组合后作用高于两者单独使用。海藻糖与磷脂间存在相互作用,该作用可能是海藻糖的-OH与磷脂PO2-形成的氢键。海藻糖和HA复配可以充分发挥两者的互补作用,即海藻糖的"水替代"作用与HA的"玻璃态"作用有机结合,因此保护效果最佳。

关键词: 海藻糖, 透明质酸, 膜脂双层, 保护作用

Abstract: According to drug retention, diameter change, glass-transition temperature (Tg) of lyophilized liposomes and the interaction of phospholipid and carbohydrates, the protective effects and mechanisms by trehalose and hyaluronic acid (HA) were examined. Bilayer fusion and drug leakage induced by lyophilization are inhibited by trehalose, lactose, sucrose, HA and the combination of trehalose and HA. The capacity of HA alone to protect liposomes is low, however, the combination of trehalose and HA is more effective than the two additives used alone. There is interaction between trehalose and phospholipid after lyophilization. This interaction is formed between -OH groups of trehalose and PO2- groups of phospholipid by hydrogen bonding. Trehalose and HA offer complementary properties when the two types of additives are used together. The combination of "water replacement" produced by trehalose and "glassy state" produced by HA results in the optimal protective effects.

Key words: trehalose, hyaluronic acid, membrane lipid bilayer, protective effect