食品科学 ›› 2008, Vol. 29 ›› Issue (8): 214-218.

• 工艺技术 • 上一篇    下一篇

酶法改性甜菊糖甙的工艺研究

郁军,岳鹏翔,程其春   

  1. 大闽国际集团研发中心
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Enzymatic Modification of Steviosides

 YU  Jun, YUE  Peng-Xiang, CHENG  Qi-Chun   

  1. Research and Development Center,Damin International Group,Zhangzhou 363000,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 本研究旨在通过α-淀粉酶和环糊精转糖苷酶(CGTase)对甜菊糖甙分子进行改性研究,以期改善甜菊糖甙的后苦味,经优化并最终获得酶法改性甜菊糖甙的生产工艺条件。本工艺的主要流程为:淀粉水解→酶法改性→脱色→树脂纯化→喷雾干燥,其中酶法改性为工艺中的关键步骤。本实验还研究了上述反应中淀粉溶液的浓度、温度、pH、时间、酶的用量及淀粉与甜菊糖甙的物料比对反应的影响。

关键词: 甜菊糖甙, 酶法改性, 环糊精转糖苷酶(CGTase), &alpha, -淀粉酶

Abstract: This study aimed to improve lingering bitterness of steviosides by enzymatically modifying steviosides with α-amylase and cyclodextrin glucanotransferase(CGTase) and to optimize the parameters of enzymatic modification process. The obtained process flow is starch hydrolysis → enzymatic modification → decoloration → purification with resin → spraydrying,where the enzymatic modification is the key step. The parameters in the above flow were investigated respectively,including concentration of starch,temperature,pH,enzymes amounts and ratio of starch to steviosides.

Key words: steviosides; enzymatic modification; cyclodextrin glucanotransferase(CGTase); &alpha, -amylase