食品科学 ›› 2008, Vol. 29 ›› Issue (8): 335-338.

• 工艺技术 • 上一篇    下一篇

野生牛肝菌多糖的分离鉴定及其抗氧化性研究

杨立红, 刘林德,钟旭生,冯培勇,蔡德华, 马崇军   

  1. 鲁东大学生命科学学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Separation and Identification of Polysaccharides from Natural Boletus and Their Antixidant Activities

YANG  Li-Hong, LIU  Lin-De, ZHONG  Xu-Sheng, FENG  Pei-Yong, CAI  De-Hua, MA  Chong-Jun   

  1. College of Life Sciences,Ludong University,Yantai 264025,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 野生牛肝菌烘干后经热水浸提,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后上DEAE-纤维素柱层析,分离得到四种多糖组分Nb-Ⅰ、Nb-Ⅱ、Nb-Ⅲ和Nb-Ⅳ。红外光谱对Nb-Ⅰ分析表明:Nb-Ⅰ为含有葡萄糖醛酸的β-D-吡喃葡聚糖。Sepharose CL-4B测得Nb-Ⅰ分子量为4.0×104,总抗氧化能力测定结果表明:牛肝菌粗多糖和Nb-Ⅰ均具有抗氧化能力,且后者高于前者。

关键词: 野生牛肝菌, 多糖, 分离鉴定, 总抗氧化性

Abstract: The crude polysaccharides of wild Natural Boletus were extracted by hot water and deposited by ethanol. DEAE Cellulose column chromatography was used for polysaccharides purification after the protein was removed by Seveg method,and four kinds of polysaccharides named as Nb-I,Nb-II,Nb-III and Nb-Ⅳ were separated. Infrared spectrum analysis showed that Nb-Ⅰis β-D-glucopyranose containing glucuronic acid,and Sepharose CL-4B method showed that molecular weight of NbI is 4.0×104. Both crude polysaccharides and Nb-Ⅰhave total antioxidation activity(T-AOC) ,and that of Nb-Ⅰis higher.

Key words: Natural Boletus, polysaccharide, separation and identification, total antioxidation activity