关键词: 苦瓜总皂甙, 提取, 纯化, AB-8大孔树脂, 加压柱层析

Abstract: The study aimed at the technology development of extration and purification of balsam pear total saponins by means of pressurized column chromatography. Four parameters including extraction temperature,extraction time,concentration of alcohol(extraction solvent) and ratio of solid weight to liquid volum were researched by orthogonal test design. Their optimized results are as follows:concentration of alcohol 80%(V/V) ,extraction temperature 80 ℃,extraction time 2 h and ratio of solid to liquid 1:20. Their effect order on extraction is,concentration of alcohol > ratio of solid to liquid > extraction time > extraction temperature. Then,the pressurized column filled with AB-8 resin was used to purify crude extracts balsam pear total saponins. Under the conditions of adsorption time 20 h,the solution flow 2 ml/min,elution time 60 min,ethanol concentration of eluant 80 % and eluant flow 2 ml/min,the purity of the total saponins is 48%.

Key words: balsam pear saponin, extraction, purification, AB-8 macroporous resin, pressurized column chromatography