食品科学 ›› 2008, Vol. 29 ›› Issue (8): 422-424.

• 生物工程 • 上一篇    下一篇

β-葡萄糖苷酶产生菌摇瓶发酵条件的研究

朱凤妹,杜彬, 刘长江,李军   

  1. 沈阳农业大学食品学院; 河北科技师范学院分析测试中心; 河北科技师范学院食品工程系
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Conditions Optimization of Shake Flask Fermentation for Producing β-glycosidase

 ZHU  Feng-Mei, DU  Bin, LIU  Chang-Jiang, LI  Jun   

  1. 1. College Food Science,Shenyang Agricultural University,Shenyang 110161,China;2.Deportment of Food Engineering,Hebei Normal University of Science and Technology,Changli 066600,China;3.Analysis and Testing Center,Hebei Normal University of Science and Technology,Changli 066600,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 本实验对产β-葡萄糖苷酶融菌株的摇瓶发酵条件进行了研究。采用正交交互试验对融合菌株发酵生产β-葡萄糖苷酶的摇瓶发酵条件进行优化。结果表明,最优摇瓶发酵条件:装液量20ml/250ml,接种量12%,发酵温度27℃,摇床转速180r/min,发酵时间7d。此条件下,β-葡萄糖苷酶活力为5.312U/ml。

关键词: &beta, -葡萄糖苷酶, 摇瓶发酵条件, 优化

Abstract: In this study,the shake flask fermentation condition for producingβ-glycosidase by fusion strains of Aspergillus niger and Aspergillus oryzae were optimized by orthogonal test. The results showed that the optimum conditions are rotational rate 180 r/min,fermentation temperature 27 ℃,inoculation amount 12%,medium volume 20 ml/250-ml shake flask and fermentation period 7 days. By these,the activity of β-glucosidase reaches 5.312 U/ml.

Key words: &beta, -glycosidase; shake flask fermentation condition; optimization