食品科学 ›› 2008, Vol. 29 ›› Issue (8): 430-432.

• 生物工程 • 上一篇    下一篇

香菇深层发酵胞外酶活力的研究

马琼, 周毅峰,张明英   

  1. 生物资源保护与利用湖北省重点实验室
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Extracellular Enzyme Activity from Lentinula edodes by Submerged Fermentation

 MA  Qiong, ZHOU  Yi-Feng, ZHANG  Ming-Ying   

  1. 1.Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province,Enshi 445000,China;2.College of Bioscience and Technology,Hubei Institute for Nationalities,Enshi 445000,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 本实验研究了香菇菌丝深层发酵过程中发酵液胞外酶纤维素酶、淀粉酶、半纤维素酶、蛋白酶活性的变化。结果表明:与碳水化合物降解相关的酶(纤维素酶、淀粉酶及半纤维素酶)活性变化趋势基本相同,在发酵的第6~8d出现第一个峰值,并于第l3~14d出现第二个峰值,且第二个峰值大于第一个峰值;蛋白酶分泌量在第8d达高峰值,随后蛋白酶活性迅速下降;菌丝生物量与发酵液胞外酶活性变化有较大的相关性关系,菌丝生物量于发酵的第13d达到最大值,与碳水化合物降解相关的酶纤维素酶、淀粉酶及半纤维素酶分泌量均在发酵的第l3~14d达最大值。

关键词: 香菇, 胞外酶, 深层发酵, 菌丝

Abstract: Activities variations of four extracellular enzymes from Lentinula edodes by submerged fermentation were studied. And the relationship between mycelium growth of Lentinula edodes and activities of the enzymes was investigated. The results showed that changing trends of activities of the enzymes degrading carbohydrate are similar basically. Activities of all the enzymes show two peak values during the whole fermentation,and the second peak value is higher than the first one. The obvious relationship between mycelium growth and enzyme activity exists. The maximum mycelium growth appears on the 14th day and the maximum activities of the enzyme degrading carbohydrate appear on the 13th to 14th day.

Key words: Lentinula edodes, extracellular enzyme, submerged fermentation, mycelium