食品科学 ›› 2008, Vol. 29 ›› Issue (8): 502-506.

• 分析检测 • 上一篇    下一篇

HPLC-ESI-MS/MS测定动物性食品中19种喹诺酮类药物残留的研究

李雅丽, 郝晓蕾, 冀宝庆,胥传来,沈崇钰, 丁涛   

  1. 江南大学食品学院; 江苏省出入境检验检疫局
  • 出版日期:2008-08-15 发布日期:2011-08-26

Simultaneous and High Sensitivity Determination of Nineteen Kinds of Quinolone Multiresidues in Animal Food by HPLC-ESI-MS/MS

LI  Ya-Li, HAO  Xiao-Lei, JI  Bao-Qing, XU  Chuan-Lai, SHEN  Chong-Yu, DING  Tao   

  1. 1.School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;2.Jiangsu Entry-Exit Inspection and Quarantine Bureau,Nanjing 210001,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 本研究建立了同时检测动物性食品中恩诺沙星、环丙沙星、诺氟沙星、氧氟沙星、氟甲喹、恶喹酸、双氟沙星、沙拉沙星、司帕沙星、丹诺沙星、氟罗沙星、马波沙星、伊诺沙星、奥比沙星、培氟沙星、萘啶酸、吡哌酸、洛美沙星和西诺沙星19种喹诺酮类药物的HPLC-ESI-MS/MS检测方法。动物组织样品用乙腈提取,采用甲酸水溶液-甲醇体系作为流动相,梯度洗脱,质谱检测器检测。方法的线性范围为0.3~50μg/kg,相关系数(r)大于0.9956,检出限为0.3μg/kg,19种喹诺酮类药物在鸡肉和鱼肉的平均回收率分别为75.3%~96.3%、79.7%~94.2%,日内和日间变异系数均小于10%。

关键词: 喹诺酮类药物, 动物性食品, HPLC-ESI-MS/MS, 多残留检测

Abstract: A multi-residue method was developed for the confirmation and quantitation of nineteen kinds of quinolones in animal food by HPLC-ESI-MS/MS. The samples were extracted with acetonitrile. The analytes were separated using 0.1% fomic acidmethanol system as the mobile phase with a linear gradient elution program,and determined by tandem mass detector. Results showed that their linear ranges are 0.3μg/kg to 50 μg/kg with correlation coefficients(r) more than 0.9956,and the limits of detection are 0.3 μg/kg. The mean recoveries for each analyte in chicken and fish range from 75.3%~96.3% and 79.7%~94.2% with relative standard deviations below 10%. The method is convenient,fast,safe,sensitive and accurate,and can be used to simultaneously analyze residual quinolones in actual samples.

Key words: quinolones, animal food, HPLC-ESI-MS/MS, multi-residue