反相高效液相色谱法同时测定映山红叶中两种三萜酸的含量

食品科学 ›› 2008, Vol. 29 ›› Issue (8): 507-509.

反相高效液相色谱法同时测定映山红叶中两种三萜酸的含量

胡振平, 黄华彩, 邹盛勤

宜春学院物理科学与工程技术学院；宜春学院继续教育学院；宜春学院生物工程研究所

出版日期:2008-08-15 发布日期:2011-08-26

Simultaneous Determination of Two Kinds of Triterpene Acids in Rhododendron simsii Planch Leaves by RP-HPLC

HU Zhen-Ping, HUANG Hua-Cai, ZOU Sheng-Qin

1.School of Physics Science and Technology,Yichun University,Yichun 336000,China;2.School of Continuing Education,Yichun University,Yichun 336000,China;3.Institute of Bioengineering,Yichun University,Yichun 336000,China

Online:2008-08-15 Published:2011-08-26

摘要/Abstract

摘要： 建立反相高效液相色谱法测定映山红叶中齐墩果酸和熊果酸的含量。采用Kromasil C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-0.2%磷酸水溶液(87:13),流速0.8ml/min,检测波长210nm,柱温28℃。齐墩果酸进样量在0.458～9.16μg范围内线性关系良好,相关系数为0.9996,平均加样回收率为99.45%;熊果酸进样量在0.516～10.32μg范围内线性关系良好,相关系数为0.9995,平均加样回收率为98.49%。齐墩果酸成分首次在映山红叶中发现。三批样品测定结果表明,此方法操作简便、准确、可行,可作为映山红叶中齐墩果酸和熊果酸含量测定的方法。

关键词: 映山红叶, 反相高效液相色谱, 齐墩果酸, 熊果酸, 光电二极管阵列检测器

Abstract: A RP-HPLC method for quantitative determination of oleanolic acid and ursolic acid in Rhododendron simsii Planch leaves was established. Kromasil C18 column(4.6 mm×250 mm,5 μm) was used as the chromatographic column,and methanol-0.2% aqueous solution of phosphoric acid(87:13) was used as the mobile phase running with a flow rate of 1.0 ml/min. The detection wavelength was 210 nm and the temperature of the column was 28 ℃. The linear rangers for oleanolic acid and ursolic acid are 0.458～9.16 μg(r=0.9996) and 0.516～10.32μg(r=0.9995),respectively. The average recoveries of the procedure are 99.45% for oleanolic acid and 98.49% for ursolic acid. Oleanolic acid is discovered for the first time in Rhododendron simsii Planch leaves. The determination results of the three batches of samples showed that the method is easy and accurate,which can be used for determination of oleanolic acid and ursolic acid in Rhododendron simsii Planch leaves.

Key words: Rhododendron simsii Planch leaves, RP-HPLC, oleanolic acid, ursolic acid, photodiode array detector

胡振平, 黄华彩, 邹盛勤. 反相高效液相色谱法同时测定映山红叶中两种三萜酸的含量[J]. 食品科学, 2008, 29(8): 507-509.

HU Zhen-Ping, HUANG Hua-Cai, ZOU Sheng-Qin. Simultaneous Determination of Two Kinds of Triterpene Acids in Rhododendron simsii Planch Leaves by RP-HPLC[J]. FOOD SCIENCE, 2008, 29(8): 507-509.

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