食品科学 ›› 2008, Vol. 29 ›› Issue (8): 520-522.

• 分析检测 • 上一篇    下一篇

HPLC法测定果冻食品中阿斯巴甜含量的研究

李皓,陈长武, 张培刚, 张敏   

  1. 吉林工程技术师范学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Determination Aspartame Content in Fruit Jelly by HPLC

 LI  Hao, CHEN  Chang-Wu, ZHANG  Pei-Gang, ZHANG  Min   

  1. Jilin Teacher’s Institute of Engineering and Ttechnology,Changchun 130052,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 目的:建立快速测定果冻食品中阿斯巴甜含量的系统分析方法。方法:对固体及半固体类别的食品采用针对性的前处理手段,选择C18柱和0.1%四氢呋喃-乙腈流动相作为分离条件,在210nm波长处进行检测。结果:本方法的最低检出限为2mg/kg,平均回收率为97.0%~99.2%,相对标准偏差为3.1%~5.3%。结论:方法简单快速,能准确的对成分复杂的食品中的阿斯巴甜进行定性定量分析。

关键词: 阿斯巴甜, 液相色谱法, 快速测定, 果冻

Abstract: Objective:To establish a systemic analysis method for rapidly determining content of aspartame in fruit jelly. Methods:The solid and semisolid foods were pretreated by special method,and then separated on C18 column with 0.1% tetrahydrofuran-acetonitrile as mobile phase and 210 nm detection wavelength. Result:The minimum detection limit is 2 mg/kg,and the average recoveries range from 97.0% to 99.2% with the relative standard deviations of 3.1%~5.3%. Conclusion:This method is simple and rapid. The aspartame in foods with complex components can be precisely analyzed with qualitative assay and quantitative assay.

Key words: aspartame, liquid chromatography, rapid determination, fruit jelly