食品科学 ›› 2008, Vol. 29 ›› Issue (8): 83-86.

• 基础研究 • 上一篇    下一篇

莲子心水提液体外抗氧化活性的研究

张敏,郑铁松, 陶锦鸿, 陈静   

  1. 南京师范大学食品科学与营养系
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Antioxidant Activities of Water Extracts from Lotus Germ in vitro

 ZHANG  Min, ZHENG  Tie-Song, TAO  Jin-Hong, CHEN  Jing   

  1. Department of Food Science and Nutrition,Nanjing Normal University,Nanjing 210097,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 本实验研究了莲子心水提液对氧自由基的清除作用及其对脂质过氧化反应(LPO)的抑制作用。结果表明:莲子心水提液对Fenton体系产生的羟自由基和邻苯三酚自氧化产生的超氧阴离子均有清除作用,半数清除量(IC50)分别为0.218g/ml和0.245g/ml;对小鼠肝、肾匀浆自发性过氧化反应有较好的抑制作用,IC50分别为3.038mg/ml和3.577mg/ml;通过紫外分光光度法测定了莲子心中抗坏血酸含量为1.132mg/g。用NBT法测定了水提取液中SOD总活性为2062.7U/g。

关键词: 莲子心水提液, 抗氧化活性, 氧自由基, 脂质过氧化

Abstract: The scavenging effect of water extracts from lotus germ on oxygen radicals and its inhibitory effect on lipid peroxidation(LPO) were studied. The results showed that the extracts can both scavenge hydroxyl free radical(·OH) produced by Fenton reaction and superoxide anion free radical(O2·) produced by oxidation of pyrogallol,and the inhibitory concentrations of 50%(IC50) are 0.218 g/ml and 0.245 g/ml respectively. They also have inhibitory effects on the spontaneous lipid peroxidation in liver and kidney homogenate,and the IC50 values are 3.038 mg/ml and 3.577 mg/ml respectively. The content of ascorbic acid in the extracts determined by ultraviolet spectrophotometry is 1.132 mg/g,and the activity of SOD determined by NBT photoredction assay is 2062.7 U/g.

Key words: water extracts from lotus germ, antioxidant activity, oxygen radicals, lipid peroxidation -