食品科学 ›› 2009, Vol. 30 ›› Issue (24): 344-346.doi: 10.7506/spkx1002-6630-200924076

• 分析检测 • 上一篇    下一篇

海藻中总砷和无机砷含量的测定

白 研,叶子明,林泽庆,朱杰科   

  1. 广东药学院公共卫生学院
  • 收稿日期:2008-12-19 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 白研 E-mail:angell_bai@163.com

Determination of Total Arsenic and Inorganic Arsenic in Marine Algae

BAI Yan,YE Zi-ming,LIN Ze-qing,ZHU Jie-ke   

  1. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
  • Received:2008-12-19 Online:2009-12-15 Published:2010-12-29
  • Contact: BAI Yan, E-mail:angell_bai@163.com

摘要:

采用湿法消解和盐酸浸提法处理样品,氢化物- 原子荧光光谱法测定各海藻中总砷和不同价态无机砷的含量。通过正交试验优化样品测定条件。在优化实验条件下,测得总砷检出限为0.05799μg/L,无机砷检出限为0.03351μg/L,三价无机砷检出限为0.05286μg/L;线性相关系数均在0.9994 以上;总砷精密度(RSD)为1.33% (n=5),无机砷RSD 为2.94% (n=5);总砷的平均加标回收率为102.95%,无机砷为103.40%。对11 种海藻样品进行测定,总砷含量在5.15~118.72μg/g 范围内;无机砷含量在1.05~89.19μg/g 范围内;三价无机砷含量在0.31~27.11μg/g 范围内。按照GB/T 2762 — 2005 和GB/T 19643 — 2005 限量指标中对无机砷的规定,本实验11 种样品中无机砷含量除紫晶藻外均超出标准限量。

关键词: 氢化物发生-原子荧光光谱法, 海藻, 无机砷

Abstract:

The samples were pretreated by wet nitration and HCl extraction,and the contents of As in marine algae were determined by HG-AFS.The analytical conditions were optimized by orthogonal designing.Under optimum conditions,the detection limit of total arsenic,inorganic arsenic,trivalent arsenic were 0.05799 μg/L,0.03351 μg/L and 0.05286 μg/L, respectively. The correlative coefficient of linear regression equation was over 0.9994 and the precisions of total arsenic and norganic arsenic were 1.33% and 2.94% (RSD, n=5), respectively . The average recoveries were 102.95% and 103.40% (n=5). The contents of total arsenic and inorganic arsenic in 11 kinds of marine algae samples varied within the range of 5.15 to 118.72 μg/g and 1.05 to 89.19 μg/g. Trivalent arsenic ranged from 0.31 to 27.11μg/g. The contents of inorganic arsenic in the 11 kinds of marine algae samples were beyond GB/T 2762 — 2005 and GB/T 19643 — 2005 standard except for amethyst algae.

Key words: hydride generation-atomic fluorescence spectrometry, marine algae, inorganic arsenic

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