食品科学 ›› 2010, Vol. 31 ›› Issue (19): 273-276.doi: 10.7506/spkx1002-6630-201019059

• 生物工程 • 上一篇    下一篇

副干酪乳杆菌对甘薯浆液中淀粉絮凝机理研究

李新华1,赵晓阳1,张荔力2   

  1. 1.沈阳农业大学食品学院
    2.辽宁医学院食品科学与工程学院
  • 收稿日期:2010-01-25 修回日期:2010-09-13 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 李新华 E-mail:lixh.syau@163.com

Flocculation Mechanism of Sweet Potato Starch by Addition of Lactobacillus paracasei-fermented Sweet Potato Slurry

LI Xin-hua1,ZHAO Xiao-yang1,ZHANG Li-li2   

  1. (1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China;
    2. College of Food Science and Engineering, Liaoning Medical University, Jinzhou 121001, China)
  • Received:2010-01-25 Revised:2010-09-13 Online:2010-10-15 Published:2010-12-29
  • Contact: LI Xin-hua1 E-mail:lixh.syau@163.com

摘要:

为探讨酸浆法分离甘薯淀粉传统工艺中的淀粉沉降分离机理,进一步制备高效生物絮凝剂。本实验采用Zeta 电位测定、粒度分析及离子键检测研究副干酪乳杆菌对甘薯淀粉絮凝沉淀的作用。结果表明:絮凝过程基于“架桥”机理,淀粉颗粒与菌体间靠离子键结合,絮凝作用使悬浊液中淀粉平均粒径减小,密度增大,导致淀粉颗粒凝聚沉降;通过热处理和酶处理实验分析菌体起絮凝作用的活性成分组成,表明副干酪乳杆菌活性成分为蛋白类物质。

关键词: 副干酪乳杆菌, 甘薯淀粉, 絮凝机理

Abstract:

The goal of this work was to explore the mechanism of sedimentation separation of starch by acidic steeping liquor method so as to provide experimental guidance for the development of highly efficient bioflocculant. The Lactobacillus paracasei-fermented sweet potato slurry and its centrifugation supernatant and resuspended precipitate were separately added with sweet potato starch, and the flocculation of sweet potato starch in the three liquors was studied by the determination of Zeta potential, granular size and ionic bond. The flocculation of sweet potato starch was based on the bridging mechanism, and starch granules were conjugated with bacterial cells through ionic bond, and after flocculation, mean starch granular size decreased and granular density increased, resulting in the aggregation and sedimentation of starch granules. Moreover, from the results of experiments on the thermal and enzymatic stability of substances with flocculating activity, proteins were the active components in Lactobacillus paracasei responsible for flocculation.

Key words: Lactobacillus paracasei, sweet potato starch, bridging mechanism

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