食品科学 ›› 2010, Vol. 31 ›› Issue (21): 232-235.doi: 10.7506/spkx1002-6630-201021052

• 生物工程 • 上一篇    下一篇

发酵谷物中产L- 赖氨酸益生菌的筛选与鉴定

骆超超,高学军* ,王青竹,于 微,刘晓飞,卢志勇,乔 彬   

  1. 东北农业大学 乳品科学教育部重点实验室
  • 收稿日期:2010-01-19 修回日期:2010-07-29 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 高学军 E-mail:gaoxj5390@sina.com
  • 基金资助:

    黑龙江省科技计划项目(GAO7B401-5)

Screening and Identification of L-lysine-producing Probiotics

LUO Chao-chao,GAO Xue-jun*,WANG Qing-zhu,YU Wei,LIU Xiao-fei,LU Zhi-yong,QIAO Bin   

  1. Key Laboratory of Dairy Science,Ministry of Education, Northeast Agricultural University, Harbin 150030, China
  • Received:2010-01-19 Revised:2010-07-29 Online:2010-11-15 Published:2010-12-29
  • Contact: GAO Xue-jun E-mail:gaoxj5390@sina.com

摘要:

为了扩展高产L- 赖氨酸菌种在食品、饲料中的应用与提高微生物发酵生产L- 赖氨酸的安全性,从面包、麻花、馒头、发面饼、大列巴、黏豆包发酵谷物的生面团及米酒、白酒曲中分离培养出66 株产L- 赖氨酸益生菌菌株。将这些菌株进行培养、发酵,并利用高效液相色谱技术检测其发酵液中L- 赖氨酸的含量,筛选出发酵液富含L- 赖氨酸的菌种两株。最后利用16S rDNA 分析及Blast 分析、特异性引物PCR 技术鉴定出这两株菌为肠膜明串球菌ATCC 8293 和德氏乳杆菌ATCC BAA-365。其发酵液中L- 赖氨酸含量分别为52.33、46.09g/L,较其他菌种相对较高。

关键词: 发酵谷物, L- 赖氨酸, 益生菌, 高效液相色谱, 16S rDNA, PCR

Abstract:

The present study aimed to expand the utilization of L-lysine-producing strains in food and animal feeds and raise the security of L-lysine production by microbial fermentation. In total, 66 strains having the ability to produce L-lysine were isolated from commercially available doughs for the making of bread, fried dough twists, steamed bread, steamed cake and Russian style bread and kojis for the making of rice wine and distilled liquor. These strains were cultured, and the quantitation of L-lysine content in medium at the end of culture was carried out using HPLC. Two of the 66 isolated strains were found to produce higher amount of L-lysine and the contents of L-lysine in the resultant fermentation broths were 52.33 g/L and 46.09 g/L respectively. Using 16S rDNA sequence and Blast analyses and specific primer PCR technique, they were identified as Leuconostoc mesenteroides ATCC 8293 and Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365.

Key words: fermented cereals, L-lysine, probiotics, high-performance liquid chromatography, 16S rDNA, specific PCR

中图分类号: