食品科学 ›› 2010, Vol. 31 ›› Issue (22): 318-321.doi: 10.7506/spkx1002-6630-201022069

• 分析检测 • 上一篇    下一篇

化橘红HPLC指纹图谱的建立

肖维强1,张超洪2,黄炳雄1,戴宏芬1,谢路斯2,王晓容1 ,*   

  1. 1.广东省农业科学院果树研究所 2.茂名市质量计量监督检测所
  • 收稿日期:2009-11-24 修回日期:2010-06-29 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 王晓容 E-mail:wang999983@126.com
  • 基金资助:

    广东省科技厅农业攻关项目(2006B20201045)

High Performance Liquid Chromatography Fingerprint of Tomentose Pummelo Peel

XIAO Wei-qiang1,ZHANG Chao-hong2,HUANG Bing-xiong1,DAI Hong-fen1,XIE Lu-si2,WANG Xiao-rong1,*   

  1. 1. Institute of Pomology, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Maoming Supervision Testing Institute of Quality and Metrology, Maoming 525011, China
  • Received:2009-11-24 Revised:2010-06-29 Online:2010-11-25 Published:2010-12-29
  • Contact: WANG Xiao-rong1 E-mail:wang999983@126.com

摘要:

为建立广东化州市特有药材化橘红的高效液相色谱(HPLC)指纹图谱,采用色谱条件为:Nucleosil C18 反相柱(150mm × 4.6mm,3μm),DAD 检测器,流动相为水- 体积分数50% 甲醇溶液+ 体积分数15%~50%乙腈溶液,三元梯度洗脱,流速为0.3~1mL/min,柱温35℃,分离波长315nm。结果表明:11 批次的化橘红提取物色谱图共有峰9 个;经“中药指纹图谱鉴别分析系统”统计分析,共有峰相对保留时间的相对标准偏差在0.3% 以下,相似度均在0.965 以上。本法符合指纹图谱的要求,可建立共有模式,作为科学评价化橘红产品质量的检测方法。

关键词: HPLC, 化橘红, 香豆素, 指纹图谱

Abstract:

In order to establish the fingerprint of HPLC analysis for tomentose pummelo (Exocarpium citri Grandis) peel, reversed-phase HPLC using Nucleosil C18 column (150 mm × 4.6 mm, 3μm) as the stationary phase, H2O-CH3OH-CH3CN as the mobile phase, triple gradient elution at the flow rate of 0.3-1 mL/min, DAD as the detector, column temperature at 35℃ and detection wavelength at 315 nm was used to determine the extract from tomentose pummelo peal. Results showed that 9 common peaks in HPLC chromatogram were observed in 11 samples. Through calculating by fingerprint analysis system of Chinese medicine, relative standard deviations (RSD) for relative retention time of common peaks were less than 0.3% and their similarity was more than 0.965. Therefore, a chromatographic fingerprint was established to provide scientific quality evaluation for tomentose pummelo peel.

Key words: HPLC, tomentose pummelo peel, coumarin, fingerprint

中图分类号: