食品科学 ›› 2011, Vol. 32 ›› Issue (14): 171-175.doi: 10.7506/spkx1002-6630-201114035

• 工艺技术 • 上一篇    下一篇

黑龙江小麦麦胚多肽的制备及抗氧化功能研究Ⅱ.超滤法精制抗氧化麦胚多肽工艺条件的优化

张洪微1,2,3,杨铭铎1,2,*,吴莹莹1,2,张 玲4,贾庆胜5   

  1. 1.哈尔滨商业大学食品工程学院 2.哈尔滨商业大学中式快餐研究发展中心博士后科研基地 3.黑龙江八一农垦大学食品学院 4.黑龙江生物科技职业学院 5.黑龙江北大荒丰缘麦业集团有限公司
  • 出版日期:2011-07-25 发布日期:2011-06-18
  • 基金资助:
    黑龙江省自然科学基金项目(C201041)

Preparation and Antioxidant Activity of Germ Peptides from Helongjiang Wheat Ⅱ: Optimization of Ultra-filtration Separation of Wheat Germ Peptides

ZHANG Hong-wei1,2,3,YANG Ming-duo1,2,*,WU Ying-ying1,2,ZHANG Ling4,JIA Qing-sheng5   

  1. (1. Institute of Food Engineering, Harbin University of Commerce, Harbin 150076, China;2. Postdoctoral Research Base of the Chinese Fast Food Research and Development Center, Harbin University of Commerce, Harbin 150076, China;3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;4. Heilongjiang Vocational College of Biology Scienece and Technology, Harbin 150025, China;5. Heilongjiang Beidahuang Fengyuan Wheat Industry Group Co. Ltd., Harbin 150076, China)
  • Online:2011-07-25 Published:2011-06-18

摘要: 采用3ku和6ku的超滤膜对麦胚多肽进行分级分离,考察3种分离产物对DPPH ·的清除能力,发现分子质量在3ku以下的麦胚多肽对DPPH ·的清除率可达75.32%,高于其他分离产物。采用响应面试验方法对麦胚多肽的超滤条件进行优化试验,结果表明,最佳超滤条件为超滤压力0.08MPa、超滤时间23min、超滤pH6.83、溶液质量分数2%,此时膜通量为4.85L/(m2 ·h)。所得分子质量小于3ku的麦胚多肽在质量分数11%时,其DPPH ·清除率为78.93%。超滤法精制的麦胚多肽具有良好的DPPH ·的清除能力。

关键词: 超滤法, 麦胚多肽, DPPH., 清除作用

Abstract: Wheat germ protein hydrolysate prepared by enzymatic hydrolysis was separated with ultra-filtration membranes with a molecular weight cutoff (MWCO) of 3 ku and 6 ku, respectively. Four ultra-filtration parameters such as pressure, time, pH and sample concentration were optimized by response surface methodology to be 0.08 MPa, 23 min, 6.83 and 2%, respectively. Under these conditions, the ultra-filtration membrane flux of was 4.85 L/(m2 ·h). The obtained fraction of less than 3 ku in molecular weight exhibited a DPPH free radical scavenging rate of 78.93% at the concentration of 11%.

Key words: wheat germ peptide, DPPH free radical scavenging activity, ultra-filtration

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