食品科学 ›› 2012, Vol. 33 ›› Issue (14): 162-165.

• 分析检测 • 上一篇    下一篇

微波消解—火焰原子吸收测定牛奶中钙的含量

孙 琦,刘 鹭,张书文,梁 霄,吕加平*   

  1. 中国农业科学院农产品加工研究所,农业部农产品加工与质量控制重点开放实验室
  • 收稿日期:2011-06-03 修回日期:2012-05-16 出版日期:2012-07-25 发布日期:2012-07-27
  • 通讯作者: 吕加平 E-mail:lvjp586@vip.sina.com
  • 基金资助:

    公益性行业(农业)科研专项

Determination of Calcium in Milk by Microwave Digestion and Flame Atomic Spectrometry

  • Received:2011-06-03 Revised:2012-05-16 Online:2012-07-25 Published:2012-07-27

摘要: 利用微波消解处理牛奶,通过火焰原子吸收光谱法测定钙的质量浓度,探讨微波消解制样技术在乳及乳制品中的应用。对微波消解过程中的硝酸体积、过氧化氢体积及消解时间分别进行单因素试验,在此基础上进行正交试验确定微波消解制样的最佳工艺参数,并对方法的准确度和回收率进行验证。结果表明:最佳消解条件为过氧化氢0.9mL、硝酸4.0mL,微波消解28min,在此条件下,经火焰原子吸收光谱法所测得钙的回收率在96.0%~98.1%之间,相对标准偏差为0.50%。微波消解处理牛奶样品耗时短,消解完全彻底,适合大规模样品的快速处理,并且提高了分析结果的精密度。

关键词: 微波消解, 火焰原子吸收, 牛奶,

Abstract: Microwave digestion technique was used in the determination of calcium in milk by flame atomic spectrometry and the application of this technique in dairy products was optimized by one-factor-at-a-time and orthogonal array design methods. The optimal digestion conditions were 0.9 mL of nitric acid, 4.0 mL of hydrogen peroxide and 28 min of digestion time. The recovery rate was in range of 96.0%-98.1% for calcium in milk with RSD of 0.50%. Our study shows that microwave digestion allows rapid and large-scale sample pretreatment is helpful to increase the accuracy of determination for calcium because of less time consumption and complete digestion.

Key words: microwave digestion, flame atomic spectrometry, milk, calcium

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