食品科学 ›› 2012, Vol. 33 ›› Issue (14): 20-24.

• 工艺技术 • 上一篇    下一篇

白骨壤叶总黄酮的超声辅助提取及其抗氧化活性研究

夏杏洲1,2,韩维栋3,杨 维1,郭远鑫1,王维民1,谌素华1   

  1. 1. 广东海洋大学食品科技学院
    2.华南理工大学轻工与食品学院 3.广东海洋大学农学院
  • 收稿日期:2010-09-17 修回日期:2012-06-18 出版日期:2012-07-25 发布日期:2012-07-27
  • 通讯作者: 夏杏洲 E-mail:xiaxzh@126.com

Ultrasonic-Assisted Extraction and Antioxidant Activity of Total Flavonoids from Avicennia marina Leaves

  • Received:2010-09-17 Revised:2012-06-18 Online:2012-07-25 Published:2012-07-27

摘要: 在单因素试验基础上,采用响应面分析法研究乙醇体积分数、固液比、超声功率和提取时间4个白骨壤叶总黄酮超声辅助提取因素,并比较白骨壤叶总黄酮的体外抗氧化作用。结果表明:白骨壤叶黄酮超声提取的最佳工艺条件为以粒度40~60目的白骨壤叶干粉为原料、乙醇体积分数64.39%、固液比1:28.20(g/mL)、提取功率293.63W、提取时间40.00min,该条件下白骨壤叶中总黄酮提取率为4.185%。白骨壤叶总黄酮体外清除羟自由基、超氧阴离子自由基和1,1-二苯基苦基苯肼(DPPH)自由基的IC50值分别0.232、0.829、23.692mg/L,其清除3种自由基的能力均高于VC。

关键词: 白骨壤叶, 黄酮, 超声辅助提取, 响应面分析法, 抗氧化活性

Abstract: On the basis of one-factor-at-a-time experiments, response surface analysis was employed to optimize four process parameters affecting the ultrasonic-assisted extraction of total flavonoids from Avicennia marina leaves including ethanol concentration, solid-to-solvent ratio, ultrasonic power and extraction time. Besides, the antioxidant activity in vitro of total flavonoids from Avicennia marina leaves was evaluated. The optimal extraction conditions were found as follows: dried Avicennia mMarina leaf powder (particle size: 40-60 mesh) was extracted with 64.39% aqueous ethanol solution at a solid-to-solvent ratio of 1:28.20 (g/mL) and a ultrasonic power of 293.63 W for 40.00 min. Under these conditions, the extraction yield of total flavonoids was 4.185%. The IC50 of the obtained extract against hydroxyl, superoxide anion and DPPH radicals were 0.232, 0.829 mg/L and 23.692 mg/L, respectively. All three types of radicals could be more effectively scavenged by the extract than VC.

Key words: Avicennia marina leaves, flavonoids, ultrasonic-assisted extraction, response surface methodology (RSM), antioxidant activity