食品科学 ›› 2012, Vol. 33 ›› Issue (14): 219-222.

• 分析检测 • 上一篇    下一篇

不同树种松花粉脂肪酸的GC-MS分析比较

陈 伟1,2,孙秀霞2,刘伟伟3,邬旭然1,*,刘永明1   

  1. 1.烟台大学化学化工学院 2.烟台经济技术开发区疾病预防控制中心 3.烟台新时代健康产业有限公司
  • 收稿日期:2011-06-08 修回日期:2012-05-29 出版日期:2012-07-25 发布日期:2012-07-27
  • 通讯作者: 邬旭然 E-mail:ytdxwxr@126.com

Comparative Determination of Fatty Acids in Pine Pollen from Different Varieties by GC-MS

Wei CHEN,XIU-xia SUN,WEI-wei LIU,XU-ran WU,YONG-ming LIU   

  • Received:2011-06-08 Revised:2012-05-29 Online:2012-07-25 Published:2012-07-27
  • Contact: XU-ran WU E-mail:ytdxwxr@126.com

摘要: 建立索氏提取-甲酯化-气相色谱-质谱联用仪分离测定松花粉中脂肪酸相对含量的方法,并用该方法对不同树种松花粉中12种主要脂肪酸相对含量进行测定与比较。结果表明:索氏提取-甲酯化-气相色谱-质谱联用是一种快速、有效的测定松花粉中脂肪酸相对含量的方法。除棕榈酸和珠光脂酸外,不同树种松花粉中其他10种脂肪酸平均相对含量均存在显著差异(P<0.05)。不同树种松花粉中饱和脂肪酸和不饱和脂肪酸平均相对含量均存在极显著差异(P<0.01)。

关键词: 松花粉, 脂肪酸, GC-MS

Abstract: A GC-MS method for the determination of fatty acids in pine pollen was established using Soxhlet extraction, methyl esterification and GC-MS and applied to comparatively analyze the relative contents of 12 main fatty acids in pone pollen from different varieties. This method provided a rapid, effective strategy for the determination of fatty acids in pine pollen. Except palmitic acid and heptadecanoic acid, all other 10 identified fatty acids showed a significant difference in their relative average contents (P <0.05). Moreover, both the relative average contents of saturated and unsaturated fatty acids exhibited a highly significant difference among five investigated varieties of pine pollens (P < 0.01).

Key words: pine pollen, fatty acid, GC-MS

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