响应面法优化葛根总黄酮的超声辅助提取工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (14): 41-44.

响应面法优化葛根总黄酮的超声辅助提取工艺

陈雅维，张延萍，周冬菊，周惠云，陈丽娜，尹卫平

河南科技大学化工与制药学院

收稿日期:2011-07-11 修回日期:2012-06-09 出版日期:2012-07-25 发布日期:2012-07-27
通讯作者: 周冬菊 E-mail:bunny_2001@163.com

Response Surface Methodology for Optimization of Ultrasound-Assisted Extraction of Flavonoids from Radix Puerariae

Received:2011-07-11 Revised:2012-06-09 Online:2012-07-25 Published:2012-07-27

摘要/Abstract

摘要： 为提高葛根总黄酮的得率，采用响应面试验设计优化超声辅助提取葛根总黄酮的工艺条件。在单因素试验基础上确定以超声时间、料液比及超声温度为3个主要因素，以总黄酮得率为响应值，根据Box-Behnken原理采用三因素三水平响应面试验设计进行优化。结果表明超声辅助法提取葛根总黄酮的最佳工艺条件为：以蒸馏水作为超声提取溶剂，超声时间43min、料液比1:10(g/mL)、超声温度68℃，总黄酮得率为5.28%，与预测得率5.36%相比，相对误差仅为1.49%。说明该模型可靠，与实际情况拟合较好。

关键词: 葛根, 总黄酮, 超声辅助提取, 响应面设计

Abstract: Response surface methodology was employed to optimize the ultrasound-assisted extraction of flavonoids from Radix Puerariae. The effects of extraction time, solid-to-liquid ratio, and temperature on the extraction yield of flavonoids were studied by one-factor-at-a-time method. A regression model for the yield of flavonoids was established using a Box-Behnken design involving the above three factors at three levels. The results showed that the optimal extraction conditions were as follows: distilled water as extraction solvent, extraction time of 43 min, solid-to-liquid ratio of 1:10 (g/mL), and temperature of 68 ℃. Under these conditions, the observed yield of flavonoids was 5.28% in comparison with the predicted yield of 5.36%, and the relative error was only 1.49%, suggesting that the optimal extraction conditions are reliable.

Key words: Radix Puerariae, flavonoids, ultrasound-assisted extraction, response surface methodology (RSM)

中图分类号:

R284.2

陈雅维，张延萍，周冬菊，周惠云，陈丽娜，尹卫平. 响应面法优化葛根总黄酮的超声辅助提取工艺[J]. 食品科学, 2012, 33(14): 41-44.

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