食品科学 ›› 2012, Vol. 33 ›› Issue (14): 50-54.

• 工艺技术 • 上一篇    下一篇

糯米藤多糖的提取工艺优化

杨 梅,王文兰,吴 昊,陈炼红,骆 斌,王晓玲*   

  1. 西南民族大学少数民族药物研究所
  • 收稿日期:2011-05-26 修回日期:2012-06-18 出版日期:2012-07-25 发布日期:2012-07-27
  • 通讯作者: 王晓玲 E-mail:wxl3232@sina.com

Optimization of Extraction Process for Polysccharides from Whole Plants of Gonostegia hirta (BL.) Miq.

  • Received:2011-05-26 Revised:2012-06-18 Online:2012-07-25 Published:2012-07-27

摘要: 目的:研究提取糯米藤多糖的最佳工艺条件。方法:以多糖提取率为考察指标,溶剂回流作为提取手段,利用单因素及正交试验确定影响提取的主要因素,然后使用回归正交组合设计优化提取工艺。结果:影响糯米藤多糖提取率的因素顺序为提取温度>液固比>提取时间>提取次数>浸泡时间;回流提取的最佳工艺条件为在96.26℃水浴条件下,提取时间109.20min、提取次数2次、液固比27.28:1(mL/g)、浸泡时间90min,最优工艺条件下多糖提取率可达25.121%。结论:使用回归正交设计能合理地优化糯米藤多糖的提取工艺。

关键词: 糯米藤, 多糖, 正交回归, 提取率

Abstract: Objective: To find optimal conditions for the extraction of polysaccharides from whole plants of Gonostegia hirta (BL.) Miq. Methods: Solvent reflux extraction was used for polysaccharide extraction. Five parameters that influence extraction efficiency were investigated using one-factor-at-a-time and orthogonal array design methods, and three main ones were optimized based on a quadratic rotation-orthogonal composite experimental design. Results: In decreasing order of their effect on extraction efficiency, five influential parameters were ranked as extraction temperature>solvent-to-solid ratio>extraction time >extraction number>soaking time. Their optimal levels were 96.26 ℃, 27.28:1 (mL/g), 109.20 min each time, 2 and 90 min, respectively, resulting in an extraction yield of 25.121%. Conclusion: Quadratic rotation-orthogonal composite experimental design permits reasonable optimization of polysaccharide extraction from Gonostegia hirta (BL.) Miq.

Key words: Gonostegia hirta (BL.) Miq., polysaccharides, quadratic rotation-orthogonal composite experimental design, extraction rate

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