儿茶素和表儿茶素异构体的高效逆流色谱分离制备

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 140-143.

儿茶素和表儿茶素异构体的高效逆流色谱分离制备

成超，尹鹭，曹学丽*，徐静

北京工商大学食品学院，食品添加剂与配料北京高校工程研究中心

收稿日期:2012-04-11 修回日期:2012-07-11 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 成超 E-mail:chengchao-56@163.com

Preparative Separation of Catechin and Epi-Catechin by High Performance Counter-Current Chromatography

Received:2012-04-11 Revised:2012-07-11 Online:2012-08-15 Published:2012-09-07

摘要/Abstract

摘要： 应用高效逆流色谱法(HPCCC)对茶叶中的儿茶素和表儿茶素两种同分异构体的分离制备方法进行研究。结果表明：以正己烷-乙酸乙酯-水(体积比1:20:20)为两相溶剂系统，上相为固定相，下相为流动相，可以实现儿茶素和表儿茶素的完全分离。采用138mL的分离柱、1g儿茶素粗提物，在120min内一次制备即可得到纯度在95%以上的表儿茶素约35mg，纯度在99%以上的儿茶素325mg，纯度90%～99%的儿茶素100mg。

关键词: 表儿茶素, 儿茶素, 异构体, 高效逆流色谱, 分离制备

Abstract: High performance counter-current chromatography (HPCCC) was used for the separation and preparation of two isomers, catechin and epicatechin from crude extract of green tea. The results indicated that catechin and epicatechin could be separated completely by HPCCC using a two-phase solvent system composed of hexane, ethyl acetate and water (1:20:20, V/V) to form an upper phase as the stationary phase and a lower phase as the mobile phase. By using a 138 mL separation column, approximately 35mg of epicatechin (＞ 95% purity) and 325mg of (＞ 99% purity) or 100mg of (90%－99% purity) cetechin were obtained from 1 g of crude extract through one-step separation in 120 min.

Key words: epicatechin, catechin, isomers, high-performance counter-current chromatography (HPCCC) , separation and purification

中图分类号:

TS201.1

成超，尹鹭，曹学丽*，徐静. 儿茶素和表儿茶素异构体的高效逆流色谱分离制备[J]. 食品科学, 2012, 33(15): 140-143.

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