食品科学 ›› 2012, Vol. 33 ›› Issue (15): 270-273.

• 营养卫生 • 上一篇    下一篇

茯苓总三萜对小鼠肝损伤的治疗作用

张先淑1,饶志刚2,胡先明2,*,刘 鹏2   

  1. 1.重庆工贸职业技术学院 2. 病毒学国家重点实验室,组合生物合成与新药发现教育部重点实验室,武汉大学药学院
  • 收稿日期:2011-07-06 修回日期:2012-07-07 出版日期:2012-08-15 发布日期:2012-09-07
  • 通讯作者: 张先淑 E-mail:czqpeng@126.com
  • 基金资助:

    国家科技部重点专项和中央高校基本科研业务费专项资金

Preventive Effect of Triterpenes from Poria cocos on Liver Injury in Mice

  • Received:2011-07-06 Revised:2012-07-07 Online:2012-08-15 Published:2012-09-07

摘要: 目的:研究茯苓三萜对四氯化碳(CCl4)所致小鼠肝损伤的治疗作用。方法:将小鼠随机分为正常组、模型组、联苯双酯组及茯苓三萜高、中、低剂量组共6组。小鼠腹腔注射CCl4橄榄油溶液造模。治疗组以高、中、低剂量(180、90、45mg/(kg·d))茯苓三萜进行灌胃,阳性药物对照组用联苯双酯滴丸(180mg/(kg·d)),皆以1g/100mL羧甲基纤维素钠溶液混悬,0.2mL/10g体质量进行灌胃;正常组与模型组以0.9%生理盐水(0.2mL/10g)体质量灌胃。最后摘眼球取血,收集血清,检测丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性;以及小鼠肝组织HE染色后做病理切片观察。结果:与模型组相比茯苓三萜治疗组能够显著降低小鼠血清中AST、ALT活性。病理切片清楚地显示茯苓三萜能够显著减轻小鼠肝损伤的程度。结论:茯苓三萜对CCl4所致的小鼠肝损伤有明显治疗作用。

关键词: 茯苓三萜, 小鼠, 肝损伤, 四氯化碳, 治疗作用

Abstract: Objective :To explore the preventive effect of triterpenes from Poria cocos on carbon tetrachloride-induced liver injury in mice. Methods: Kunming mice were randomly divided into normal group, model group,  (dimethyl diphenyl bicarboxylate,DDB)  positive control group, and low-, middle- and high-dose treatment groups. Mouse models of liver injury were created by intraperitoneal injection of CCl4. The high-, middle- and low-dose treatment groups were orally administered with triterpenes from Poria cocos at doses of 180, 90 mg/(kg · d) and 45 mg/(kg·d), respectively. The positive control group was orally administered with DDB at a dose of 180 mg/(kg·d). Both drugs were separately suspended in 1 g/100 mL CMC-Na solution before administration and given at a level of 0.2 mL/10 g body weight. The normal and model groups were given 0.9% normal saline at a level of 0.2 mL/10 g body weight. The serum AST and ALT activities were determined and liver injury was evaluated by HE staining. Results: Compared with the model group, the three treatment groups showed significantly lower AST and ALT activities. In addition, Poria cocos triterpenes could significantly attenuate liver injury in mice. Conclusion:  Poria cocos triterpenes have obvious protective effect on CCl4-induced liver injury in mice.

Key words: Poria cocos triterpenes, mice, liver injury, carbon tetrachloride, treatment efficacy

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