草鱼活性肽体外生理功能

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 34-37.

草鱼活性肽体外生理功能

林利美1，陈季旺1，吴永宁2，宫智勇1,*

1.武汉工业学院食品科学与工程学院 2.国家食品安全风险评估中心

收稿日期:2012-05-15 修回日期:2012-07-10 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 宫智勇 E-mail:zhiyong_gong@yahoo.com.cn
基金资助:
国家“863”计划项目

in vitro Physiological Functions of Bioactive Peptides from Grass Carp Meat

Received:2012-05-15 Revised:2012-07-10 Online:2012-08-15 Published:2012-09-07

摘要/Abstract

摘要： 以草鱼为原料制取生理活性肽，采用高效液相色谱法(HPLC)测定其对血管紧张素转化酶(ACE)的抑制活性，采用DPPH自由基清除法、氧自由基清除能力(ORAC) 法研究其抗氧化性。结果表明：草鱼肽抑制ACE活性IC50为0.229mg/mL，清除DPPH自由基的IC50为7.630mg/mL，ORAC值为1317.962μmol Trolox/g，采用草鱼制备有效的生理活性肽是可行的。

关键词: 草鱼肽, ACE抑制活性, DPPH自由基, ORAC

Abstract: In this study, bioactive peptides were prepared from grass carp meat by enzymatic hydrolysis and ultrafiltration. Their angiotensin I-converting enzyme (ACE) inhibitory activity was determined by high performance liquid chromatography (HPLC) and antioxidant activity was evaluated by DPPH radical scavenging and ORAC (oxygen radical absorbance capacity) assays. The results showed that the prepared bioactive peptides had strong ACE inhibitory activity with IC50 of 0.229 mg/mL. The concentration needed to scavenge 50% DPPH radicals was 7.630 mg/mL and an ORAC value of 1317.962 Trolox μmol/g was achieved. Thus, grass carp meat is a good source of ACE inhibitory peptides.

Key words: grass carp peptides, ACE inhibitory activity, DPPH free radical, ORAC

中图分类号:

TS254.9

林利美1，陈季旺1，吴永宁2，宫智勇1,*. 草鱼活性肽体外生理功能[J]. 食品科学, 2012, 33(15): 34-37.

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