超临界CO2密度对全反式番茄红素吸光系数的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 52-56.

超临界CO2密度对全反式番茄红素吸光系数的影响

何强强，惠伯棣*，宫平

北京联合大学应用文理学院

收稿日期:2011-07-04 修回日期:2012-05-11 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 惠伯棣 E-mail:bodi_hui@ygi.edu.cn
基金资助:
“十一五”国家科技支撑计划项目

Effect of Supercritical Carbon Dioxide Density on Absorption Coefficient of All-trans Lycopene

Received:2011-07-04 Revised:2012-05-11 Online:2012-08-15 Published:2012-09-07
Contact: Bodi HUI E-mail:bodi_hui@ygi.edu.cn

摘要/Abstract

摘要： 目的：通过在超临界CO2色谱上测定CO2流动相密度与全反式番茄红素组分峰面积的相关性，推算二氧化碳密度变化对全反式番茄红素的吸光系数(A1cm)的影响。方法：超临界色谱条件：色谱柱：Diamonsil C8(250mm×4.6mm，5μm)；检测波长：453nm；压力变化范围：11.5～17.5MPa；温度变化范围：35～55℃；流速：2mL/min；进样量：20μL。结果：全反式番茄红素组分在超临界CO2中最大吸收波长处的峰面积是可变的，与CO2密度呈线性正相关。结论：可推断全反式番茄红素吸光系数与超临界CO2流体密度变化呈正相关。

关键词: 全反式番茄红素, 超临界流体色谱, 超临界CO2, 密度, 吸光系数(A1cm)

Abstract: Objective: To assess the effect of varying carbon dioxide density on absorption coefficient (A1cm) of all-trans lycopene through exploring the correlation between carbon dioxide density in supercritical fluid chromatography (SFC) and peak areas of all-trans lycopene fractions. Methods: Supercritical CO2 fluid chromatography was carried out on a Diamonsil C8 (250mm × 4.6 mm, 5 μm, ) at a pressure ranging from 11.5 to 17.5 MPa, and a temperature ranging from 35—55 ℃. The detection wavelength was 453 nm, and 20 μL sample was injected for SFC chromatography. Results: Peak area of all-trans lycopene at the maximum absorption wavelength in supercritical carbon dioxide fluid chromatography revealed a variation and was linearly positive correlated with the density of carbon dioxide. Conclusion: Absorption coefficient of all-trans lycopene is positively correlated with the density of supercritical carbon dioxide.

Key words: all-trans lycopene, supercritical fluid chromatography (SFC), supercritical carbon dioxide, density, absorption coefficient (A1cm)

何强强，惠伯棣*，宫平. 超临界CO2密度对全反式番茄红素吸光系数的影响[J]. 食品科学, 2012, 33(15): 52-56.

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