食品科学 ›› 2012, Vol. 33 ›› Issue (2): 173-176.doi: 10.7506/spkx1002-6630-201202037

• 分析检测 • 上一篇    下一篇

离子色谱法直接测定食品添加剂中的甜蜜素

朱怀远,庄亚东,熊晓敏,万 敏,沈晓晨,张 映   

  1. 江苏中烟工业有限责任公司技术研发中心
  • 收稿日期:2017-02-16 修回日期:2017-02-16 出版日期:2012-01-25 发布日期:2012-01-16

Direct Determination of Sodium Cyclamate in Food Additives by Ion Chromatography

ZHU Huai-yuan,ZHUANG Ya-dong,XIONG Xiao-min,WAN Min,SHEN Xiao-chen,ZHANG Ying   

  1. Technology and Research Center, China Tobacco Jiangsu Industrial Co. Ltd., Nanjing 210019, China
  • Received:2017-02-16 Revised:2017-02-16 Online:2012-01-25 Published:2012-01-16

摘要: 建立离子色谱法直接测定食品添加剂中的甜蜜素的方法。利用甜蜜素具有较大水溶性、在水中易电离的特性,采用去离子水振荡提取样品中的甜蜜素,30mmol/L氢氧化钾溶液作为淋洗液,经阴离子交换色谱柱分离,电导检测器-离子色谱法直接测定甜蜜素。结果表明:甜蜜素含量在5~200μg/mL范围内,方法的线性关系良好(相关系数为0.9999),加标回收率为95.54%~99.24%,检出限为0.087μg/mL,方法的日内相对标准偏差小于3%,日间相对标准偏差小于2%。方法简便、稳定性好,不需对样品进行复杂的预处理,可实现对食品添加剂中甜蜜素的便捷、快速、准确测定。

关键词: 离子色谱法, 食品添加剂, 甜蜜素

Abstract: A new method was developed for direct determination of sodium cyclamate in food additives by ion chromatograph coupled with electrical conductivity detector based on high water solubility and ionization characteristics of sodium cyclamate. Sodium cyclamate in samples were extracted into deionized water by shaking, separated on an anion exchange column using 30 mmol/L KOH aqueous solution as elution solvent and detected by an electrolytic conductivity detector. The method showed good linearity over the concentration range of 5-200 μg/mL with a correlation coefficient of 0.9999 and a detection limit of 0.087 μg/mL. The intraday and inter-day relative standard deviations (RSDs) were less than 3% and 2% respectively. The method was simple and stable without complex sample pretreatment, and allowed easy, rapid and accurate determination of sodium cyclamate in food additives.

Key words: ion chromatography, food additives, sodium cyclamate

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