食品科学 ›› 2012, Vol. 33 ›› Issue (20): 282-285.

• 分析检测 • 上一篇    下一篇

催化动力学光度法测定茶叶中硒浸出率

赖海涛1,苏国成1,2,*,张锦龙1   

  1. 1.集美大学生物工程学院 2.厦门市食品科技研发检测中心
  • 收稿日期:2011-09-01 修回日期:2012-08-25 出版日期:2012-10-25 发布日期:2012-11-09
  • 通讯作者: 赖海涛 E-mail:lht0592@jmu.edu.cn
  • 基金资助:

    厦门市重大科技创新平台项目

Determination of Leaching Rate of Selenium from Tea by Catalytic Kinetic Spectrophotometry

  • Received:2011-09-01 Revised:2012-08-25 Online:2012-10-25 Published:2012-11-09

摘要: 在盐酸介质中,硒(Ⅳ)能强烈催化溴酸钾氧化亚甲基蓝使之褪色,据此建立测定痕量硒(Ⅳ)的催化动力学光度法,在选定的条件下,硒(Ⅳ)的测定线性范围为0~0.6μg/50mL,检出限3.63×10-10g/mL。本实验通过催化动力学光度法可测出茶叶中原茶及冲泡后硒(Ⅳ)含量,计算可得茶叶中硒(Ⅳ)浸出率。通过对铁观音、绿茶、红茶进行测定,其浸出率分别为24.7%、25.3%、22.7%,结果令人满意。

关键词: 茶叶, 硒(Ⅳ), 浸出率, 催化动力学光度法 

Abstract: In hydrochloric acid medium, Se (Ⅳ) powerfully catalyzes the oxidation of methylene blue by potassium bromate, causing methylene blue to fade. Based on this, a new catalytic kinetic spectrophotometric method was developed in this study for determining trace Se (Ⅳ). Under given conditions, the linear range for Se (Ⅳ) determination was 0–0.6 μg/50 mL. The limit of detection of the developed method was 3.63×10-10 g/mL. This method allowed the determination of Se (Ⅳ) content in original and infused tea so that the leach rates of Se (Ⅳ) from Tieguanyin, Green tea and Black tea in hot water could be calculated from both measured values as being 24.7%, 25.3% and 22.7%, respectively.

Key words: tea, Se (Ⅳ), leaching rate, catalytic kinetic spectrophotometry

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