基于酶法辅提和微波酸解的黄姜薯蓣皂苷元清洁生产工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 94-98.

基于酶法辅提和微波酸解的黄姜薯蓣皂苷元清洁生产工艺

李会，张佳佳，梁静怡，许正宏，史劲松*

江南大学医药学院

收稿日期:2012-04-06 修回日期:2012-09-06 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 史劲松 E-mail:shijs@163.com
基金资助:
国家“863”计划;中央高校基本科研业务费专项资金资助

Clean Production of Diosgenin from Dioscorea zingiberensis C. H. Wright Based on Enzymatic Treatment and Microwave-Assisted Acidolysis

Received:2012-04-06 Revised:2012-09-06 Online:2012-10-25 Published:2012-11-09

摘要/Abstract

摘要： 根据清洁生产以及资源综合利用的理念，研究了一种从盾叶薯蓣根茎中提取皂苷元的新工艺。此工艺包括4个主要步骤，即原料中淀粉、纤维素的回收，复合酶水解，微波酸解和皂苷元提取。首先利用物理方法使大部分的半纤维素和淀粉从块茎分离，该过程引入超声洗涤减少用水量。第2步，加入耐高温α-淀粉酶和糖化酶进一步酶解残留的淀粉及半纤维素。第3步，采用酸水解释放薯蓣皂苷元，该过程引入微波加热方式减少酸用量并缩短酸解时间。最后是使用少量溶剂提取皂苷元并通过重结晶进行精制。结果表明：与传统直接酸水解法相比，采用以上清洁生产工艺，酸的用量减少96.97%，薯蓣皂苷元的得率提高34.56%，实现了黄姜皂苷元的清洁生产。

关键词: 复合酶, 微波, 黄姜, 皂苷元, 清洁生产

Abstract: Based on the concept of clean production and comprehensive resource utilization, a new procedure for the extraction of diosgenin from Dioscorea zingiberensis C. H. Wright rhizomes was proposed. This procedure involved four stages, recovery of starch and cellulose from the raw material, one-step multi-enzymatic hydrolysis, microwave-assisted acidolysis and diosgenin extraction. First, most of the starch and cellulose in Dioscorea zingiberensis C. H. Wright rhizomes were extracted physically. In this process, ultrasonication was introduced to save water. Second, the residual starch and cellulose were further hydrolyzed with added thermostable α-amylase and glucoamylase. Third, diosgenin was released by acidolysis. In this process, microwave heating was used to save acid and shorten the acidolysis time. Finally, a small quantity of solvent was used to extract diosgenin and the extract was refined by re-crystallization. Compared with the traditional single acidolysis procedure, this clean production procedure could save acid by 96.97% and result in an increase in diosgenin yield of 34.56%.

Key words: multi-enzyme, microwave, Dioscorea zingiberensis C. H. Wright, diosgenin

中图分类号:

S567.23+9

李?会，张佳佳，梁静怡，许正宏，史劲松*. 基于酶法辅提和微波酸解的黄姜薯蓣皂苷元清洁生产工艺[J]. 食品科学, 2012, 33(20): 94-98.

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