食品科学 ›› 2012, Vol. 33 ›› Issue (21): 20-23.

• 基础研究 • 上一篇    下一篇

磁场对苦荞种子萌发过程中黄酮类物质的诱导效应

周小理,方 向,周一鸣,欧阳倩,吕 喆,马 珺   

  1. 上海应用技术学院香料香精技术与工程学院
  • 收稿日期:2012-06-05 修回日期:2012-09-21 出版日期:2012-11-15 发布日期:2012-11-09
  • 通讯作者: 周小理 E-mail:zhouxl@sit.edu.cn
  • 基金资助:

    荞麦萌发期黄酮类生物活性物质的调控及其免疫功能研究

Effect of Magnetic Field Stimulation on Flavonoid Synthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Sprouts

  • Received:2012-06-05 Revised:2012-09-21 Online:2012-11-15 Published:2012-11-09

摘要: 研究不同磁场强度诱导下,苦荞种子萌发过程中苯丙氨酸解氨酶(PAL)、查尔酮异构酶(CHI)及芦丁降解酶(RDEs)3种关键酶的酶比活力变化规律及与总黄酮含量的关系。结果表明:1)不同磁场强度对同一种酶的诱导效应不同:PAL酶比活力在磁场强度为0.3T时达到最高值;CHI酶比活力最高值出现在磁场强度为0.2T时;当磁场强度为0.3T时,RDEs酶比活力与对照相比无显著性差异;2)相同磁场强度对不同酶的诱导效应不同:强度为0.3T时,PAL酶比活力变化最大;强度为0.2T时,CHI酶比活力变化最大;3)当磁场强度为0.3T时,3种酶的协同作用使苦荞萌发物中总黄酮含量达到最高值(62.90mg/g);实验证实磁场对苦荞种子萌发过程中黄酮类物质的增加具有一定诱导效应。

关键词: 磁场处理, 苦荞萌发物, 酶比活力, 总黄酮

Abstract: In this study, tartary buckwheat seeds were pretreated by different magnetic field intensities. During subsequent germination, the changes in the specific activity of three key enzymes including phenylalanine ammonia lyase (PAL), chalconeisomerase (CHI) and rutin-degrading enzymes (RDEs) and their relationships with total flavonoid content were explored. The results showed that (1) the same enzyme was induced to different extents by different magnetic field intensities: the biggest specific activity of PAL was attained at 0.3 T, whereas the specific activity of CHI reached the highest level at 0.2 T, and no significant difference in the specific activity of RDEs was observed when compared with the control; 2) the same magnetic field intensity induced different enzymes to different extents: PAL showed the biggest variation in its specific activity at 0.3 T, while the biggest variation was observed for CHI at 0.2 T; (3) the highest level (62.90 mg/g) of total flavonoids in germinated tartary buckwheat seeds was reached in response to the combined effect of three enzymes at 0.2 T. This study demonstrates that magnetic field stimulation can induce the production of flavonoids in tartary buckwheat seeds during germination.

Key words: magnetic field, tartary buckwheat (Fagopyrum tataricum Gaertn.) sprouts, enzyme specific activity, total flavonoids

中图分类号: