大豆异黄酮对AD大鼠海马PKG和Raf-1的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 314-317.

大豆异黄酮对AD大鼠海马PKG和Raf-1的影响

蔡标，彭代银*，汪远金，王艳，刘长安，宋睿，汪天明

安徽中医学院

收稿日期:2011-10-18 修回日期:2012-11-01 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 彭代银 E-mail:pengdaiyin@163.com
基金资助:
安徽省科技厅年度重点项目

Influence of Soybean Isoflavone on PKG and Raf-1 Expression in the Hippocampus of Rats with Alzheimer’s Disease

Received:2011-10-18 Revised:2012-11-01 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 目的：通过观察大豆异黄酮(SIF)对阿尔茨海默病(AD)大鼠海马组织蛋白激酶G(PKG)和Raf-1蛋白的影响，探讨大豆异黄酮治疗阿尔茨海默病作用机制。方法：采用β-淀粉样蛋白(Aβ)双侧海马注射建立AD大鼠模型，分组后给予不同剂量大豆异黄酮，Morris水迷宫实验观察大鼠学习记忆能力的变化，ELISA法测定大鼠海马组织PKG含量，免疫组化SP法观察大鼠海马组织Raf-1的表达。结果：大豆异黄酮可改善AD大鼠的学习记忆能力(P＜0.05)，显著降低AD大鼠海马组织PKG的含量(P＜0.05)，显著降低AD大鼠海马组织Raf-1的表达(P＜0.05)。结论：大豆异黄酮可能通过降低AD大鼠海马PKG的含量和Raf-1的表达起到治疗AD的作用。

关键词: 大豆异黄酮, 阿尔茨海默病, PKG, Raf-1

Abstract: Objective: To explore the effect and possible mechanism of action of soybean isoflavone (SIF) on PKG and Raf-1 expression in the hippocampus of rats with Alzheimer’s disease (AD). Method: Female Wistar rats were randomly divided into shame operation model control and SIF high, medium and low-dose groups. AD rat models were established by bilateral hippocampus injection of Aβ. Learning-memory abilities in AD rats were observed by Morris water maze test. PKG content in the hippocampus was measured by ELISA and Raf-1 expression in the hippocampus of rats was detected by immunohistochemistrical SP method. Result: SIF significantly improved learning-memory abilities (P＜0.05) and decreased PKG content and Raf-1 expression in the hippocampus of AD rats (P＜0.05). Conclusion: SIF has anti-AD effects probably by decreasing PKG content and Raf-1 expression in the hippocampus of AD rats.

Key words: soybean isoflavone, Alzheimer’s disease, PKG, Raf-1

中图分类号:

R285.5

蔡?标，彭代银*，汪远金，王?艳，刘长安，宋?睿，汪天明. 大豆异黄酮对AD大鼠海马PKG和Raf-1的影响[J]. 食品科学, 2012, 33(23): 314-317.

参考文献

[1] 汪远金，许金林，张杰等.大豆异黄酮对卵巢大鼠骨质疏松的实验研究[J].食品科学，2003，24（2）：120-122.
[2] 蔡标,申国明,汪远金等.大豆异黄酮对AD大鼠海马Apo-E4的影响[J]. 辽宁中医药大学学报，2011, 13(7):117-119.
[3] 蔡标，彭代银，汪远金等.大豆异黄酮对AD大鼠海马NO-cGMP信号转导系统的影响[J]. 中国中药杂志， 2011, 36(2):29-32.
[4] 李仪奎.中药药理实验方法学[M]第二版.上海:上海科技出版社，2006:293-294.
[5] 王维治.神经病学[M].北京：人民卫生出版社，2008:1397-1400.
[6] Weldon DT, Rogers SD, Ghilardi JR,et al. Fibrillar beta-amy-loid induces microglial phagocytosis, expression of induciblenitric oxide synthase, and loss of a select population of neu-rons in the rat CNS in vivo[J]. J Neurosci, 1998,18:2161-2173.
[7] Danson TM, Gonzalez-zulueta M, Kusel J. Nitric oxide: diverse actions in the central and peripheral nervous systems[J]. Neuroscientist, 1998,4:96-142.
[8] Khan TK, Alkon DL. An internally controlled peripheral biomarker for Alzheimer’s desease:ERK1 and ERK2 responses to the inflammatory signal bradykinin[J]. Proc Natl Acad Sci U S A,2006,29,103(35):13203-13207.
[9] Delgado M, Varela N, Gonzalez-Rey E. Vasoactive intestinal peptide protects against beta-amyloid-induced neurodegeneration by inhibiting microglia activation at multiple levels[J]. Glia, 2008,1,56(10):1091-103.
[10]Tong L, Balazs R, Soiampornkul R, et al. Transforming growth factor-beta 1 impairs endothelin-1-mediated contraction of brain vessels by inducing mitogen-activated protein (MAP) kinase phosphatase-1 and inhibiting p38 MAP kinase[J]. Mol Pharmacol, 2007,72(6):1476-83.
[11]Haskell SG, Richardson ED, Horwitz RI, et al. The effect of estrogen replacement therapy on cognitive function in women:a critical review of the literature[J]. J Clin Epidemiol, 1997, 50(11): 1249-1264.
[12]Morinaga A, Hirohata M, Ono K, et al. Estrogen has anti-amyloidogenic effects on Alzheimer's beta-amyloid fibrils in vitro[J]. Biochem Biophys Res Commun(S0006-291X), 2007, 359(3):697-702.
[13]Greenfield JP, Leung LW, Cai D.Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans -Golgi network vesicle biogenesis[J]. J Biol Chem, 2002,277(14):12128 -12136.
[14]吕海燕，贾建平.雌激素治疗阿尔茨海默病的争议[J].中国临床康复,2005,9(13):125-127.
[15]Reiter E, Beck V, Medjakovic S, et a1. Isoflavones are safe compounds for therapeutical applications-Evaluation of in vitro data[J]. Gynecol Endocrinol, 2009, 7(7): 1-27.