填饲对鹅肥肝脂肪酸合成酶基因mRNA表达的影响

doi:10.7506/spkx1002-6630-201205036

食品科学 ›› 2012, Vol. 33 ›› Issue (5): 165-169.doi: 10.7506/spkx1002-6630-201205036

填饲对鹅肥肝脂肪酸合成酶基因mRNA表达的影响

刘振春，郭炫，杨童奥，梁国朋，吴伟

吉林农业大学食品科学与工程学院

出版日期:2012-03-15 发布日期:2012-03-03
基金资助:
吉林省科技厅科技发展计划重点项目(2009026)

Effect of Feeding Period on Expression of FAS mRNA in Fatty Liver of Goose

LIU Zhen-chun，GUO Xuan，YANG Tong-ao，LIANG Guo-peng，WU Wei

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

Online:2012-03-15 Published:2012-03-03

摘要/Abstract

摘要： 以朗德鹅为对象，采用实时荧光定量PCR法研究不同填饲周龄脂肪酸合成酶基因(FAS基因)表达的规律性。结果显示，FAS基因相对表达量(FAS与β-actin 基因表达量的比值)在填饲前、填饲1周、填饲2周、填饲3周和填饲4周相对表达量逐渐增高，分别为0.035、0.128、0.253、0.876、1.009，说明肥肝组织中FAS基因表达丰度与填饲期呈显著正相关，FAS基因在鹅肥肝形成过程及体脂沉积过程中具有一定的调控作用，为促进鹅肝内脂肪沉积的主要调控基因。

关键词: 填饲, 鹅肥肝, 脂肪酸合成酶, 基因表达

Abstract: Landes geese were used as the materials to explore the expression rule of FAS mRNA in fatty liver through real-time fluorescence quantitative PCR method during overfeeding weeks. Fluorescence quantitative PCR results showed that the expression of FAS gene revealed an obvious increase during pre-feeding fold and pro-feeding 1 week, feeding 2 weeks, feeding 3 weeks and feeding 4 weeks, which were 0.035, 0.128, 0.253, 0.876 fold and 1.009 fold enhancement, respectively. A positive genetic correlation between FAS mRNA expression in fatty liver tissue and feeding weeks was observed. These results suggested that FAS gene had a certain readjustment function during the developing process of fatty liver in goose and the process of body fat deposition. Thus, FAS is a regulation gene to promote fat deposit inside liver of goose, which provides valuable technical parameters for future studies of fatty liver.

Key words: feeding, fatty liver of goose, fatty acid synthase, gene expression

中图分类号:

TS251.1

刘振春，郭炫，杨童奥，梁国朋，吴伟. 填饲对鹅肥肝脂肪酸合成酶基因mRNA表达的影响[J]. 食品科学, 2012, 33(5): 165-169.

LIU Zhen-chun，GUO Xuan，YANG Tong-ao，LIANG Guo-peng，WU Wei. Effect of Feeding Period on Expression of FAS mRNA in Fatty Liver of Goose[J]. FOOD SCIENCE, 2012, 33(5): 165-169.

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填饲对鹅肥肝脂肪酸合成酶基因mRNA表达的影响

Effect of Feeding Period on Expression of FAS mRNA in Fatty Liver of Goose

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