食品科学

• 技术应用 • 上一篇    下一篇

DNA条形码技术在鱼肉及其制品鉴别中的应用

李新光,王 璐,赵 峰,马丽萍,孙 永,周德庆   

  1. 1.中国水产科学研究院黄海水产研究所,山东 青岛 266071;2.上海海洋大学食品学院,上海 201306;
    3.中国海洋大学食品科学与工程学院,山东 青岛 266003
  • 出版日期:2013-09-25 发布日期:2013-09-27

Application of DNA Barcoding to Identify Commercial Fish and Fish Products

LI Xin-guang,WANG Lu,ZHAO Feng,MA Li-ping,SUN Yong,ZHOU De-qing   

  1. 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    3. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Online:2013-09-25 Published:2013-09-27

摘要:

为鉴别冷冻鱼、冻鱼片和烤鱼片中鱼肉成分的来源,用以判别其与食品标签是否相符,以COI基因为靶点,利用基因克隆和生物信息学方法建立鱼肉制品DNA条形码鉴别技术。对20种冷冻鱼、10种冻鳕鱼片和15种烤鱼片样品进行鉴定,结果表明:20种冷冻鱼鉴定的结果与形态学鉴定的结果一致;10种冻鳕鱼片样品主要以“狭鳕”为主(6/10),存在将“白鳕鱼”标识为“银鳕鱼”的现象;烤鱼片样品与其标签上所标识的原料多数不符,一些烤鱼片还检有月尾兔头鲀。DNA条形码技术是一种简单、有效的分子鉴别技术,可用于冷冻鱼、冻鱼片和烤鱼片中鱼肉成分的鉴定。

关键词: DNA条形码, 物种鉴别, 冻鳕鱼片, 烤鱼片

Abstract:

A DNA barcoding technique for species identification of fish ingredients in frozen whole fish, frozen
fish fillets and grilled fish fillets by using COI gen as the target was established for use in examining whether the
identified species are in accordance with those indicated on the label or not. Twenty fresh fishes, ten frozen cod
fillets and fifty grilled fish fillets were identified using the DNA Barcoding technique. The results showed that
DNA barcoding identification of the twenty frozen fishes was identical to morphological identification. Out of the
ten frozen cod fillets, six samples were identified as Theragra chalcogrammus and some samples of Merluccius
productus were labeled as Anoplopoma fimbria. Most of the grilled fish fillets were identified to be not the same
as indicated on the label and Lagocephalus lunaris ingredients were detected in some of them. DNA barcoding as a
simple and effective molecular identification approach is useful for the identification of fish ingredients in frozen
whole fish, frozen fillets and grilled fish fillets.

Key words: DNA barcoding, species identification, frozen cod fillets, grilled fish fillets