食品科学 ›› 2013, Vol. 34 ›› Issue (2): 52-57.

• 工艺技术 • 上一篇    下一篇

米糠谷蛋白干法糖基化改性的研究

那治国1,马永强2,韩春然2   

  1. 1. 黑龙江东方学院
    2. 哈尔滨商业大学
  • 收稿日期:2012-07-09 修回日期:2012-12-22 出版日期:2013-01-25 发布日期:2013-01-15
  • 通讯作者: 那治国 E-mail:nazhiguo1980@126.com
  • 基金资助:
    黑龙江省科技攻关课题

Glycosylation Modification of Rice Bran Glutelin by Dry-Heating

  • Received:2012-07-09 Revised:2012-12-22 Online:2013-01-25 Published:2013-01-15

摘要: 对米糠谷蛋白干法糖基化改性的反应条件进行了研究,确定了最佳反应条件:pH7.8,50℃,底物配比(米糠谷蛋白/葡聚糖)1:5,反应时间24h,相对湿度79%,此条件下产物的接枝度为25.67%,褐变指数为0.795。与米糠谷蛋白的功能性质进行比较,发现糖基化产物的溶解性在整个pH范围内均有提高,pH6.5时提高了82.1%;pH<8时,糖基化产物的乳化性明显升高,pH5.0时提高了3.5倍;糖基化产物起泡性改善不明显。傅立叶红外光谱分析表明米糠谷蛋白以共价键的结合形式接入了糖分子,扫描电子显微镜研究发现米糠谷蛋白呈颗粒状态,糖基化产物为薄片状结构。

Abstract: Rice bran glutelin was modified by glycosylation under dry -heating condition, and the optimal reaction conditions were: pH 7.8, 50 ℃, at 1:5 ratio of rice bran glutelin to glucose in weight, reaction time 24h, relative humidity 79,Under this condition the degree of grafting was 25.67% and index of browning was 0.795. Compared with the unmodified rice bran glutelin, the solubility of the modified rice bran glutelin had higher solubility within all the examined range of pH value. Its solubility was increased by 3.5 times at pH 5.0, but increased by 82.1% at pH 6.5. AT pH<8, emulsifying properties of the glycation products markedly was elevated, but the foaming properties of the glycation products improvement was not obvious. It was proved by FTIR that the rice bran glutelin was grafted with the glucose by the covalent bond. In addition, it was observed with SEM that the surface structure of the rice bran glutelin was different from the aggregation , which was pieces formed.

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