食品科学

• 分析检测 • 上一篇    下一篇

超高效液相色谱法检测菜籽油中的酚酸

郑 畅,杨 湄,黄凤洪,周 琦,曹晓玮   

  1. 中国农业科学院油料作物研究所,油料脂质化学与营养湖北省重点实验室,湖北 武汉 430062
  • 出版日期:2013-10-25 发布日期:2013-09-28

Determination of Phenolic Acids in Rapeseed Oil Using Ultra Performance Liquid Chromatography

ZHENG Chang,YANG Mei,HUANG Feng-hong,ZHOU Qi,CAO Xiao-wei   

  1. Hubei Key Laboratory of Lipid Chemistry and Nutrition, Institute of Oilcrops Research,Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

通过优化固相萃取(SPE)提取纯化菜籽油中酚酸的参数以及超高效液相色谱(UPLC)分离条件,建立了基于UPLC分离菜籽油中酚酸的测定方法。结果表明:利用该方法可同时对菜籽油中的芥子碱、咖啡酸、芥子酸、阿魏酸、肉桂酸5种酚酸进行高效分离;采用外标法建立了5种酚酸的定量方法,5种酚酸的标准物质在各自质量浓度范围内具有良好线性关系,相关系数为0.9994~0.9999,检出限为0.07~0.11mg/L,平均回收率为100.6%~106.7%,相对标准偏差(RSD)不大于2.9%。该方法具有杂质干扰小、回收率和灵敏度高等特点。采用该方法可用于测定高、中、低不同芥酸含量的冷榨菜籽油中酚酸含量,方差分析表明不同冷榨菜籽油中的酚酸含量具有显著性差异。

关键词: 酚酸, 菜籽油, 超高效液相色谱法, 固相萃取

Abstract:

An ultra performance liquid chromatography (UPLC) method for the determination of phenolic acids in rapeseed
oil was established by optimizing solid phase extraction parameters and chromatographic separation conditions. Five
phenolic acids such as sinapine, caffeic acid, sinapic acid, ferulic acid and cinnamic acid could be effectively separated by
this method. Quantification was performed by an external standard method. A good linear relationship was observed for five
phenol acid standards with correlation coefficients of 0.9994 to 0.9999. The detection limits were in the range of 0.07
to 0.11 mg/L. The average recovery rates of five phenolic acids in rapeseed oil were 100.6% to 106.7% with relative standard
deviations less than 2.9%. The method had the advantages of less interference from impurities and high recovery and
sensitivity. Phenolic acid composition of cold-pressed rapeseed oil (CPRO) with high, medium and low contents of erucic
acid was determined by using this method, and analysis of variance showed significant differences in the contents of phenolic
acids among high, medium and low-erucic acid CPROs.

Key words: phenolic acid, rapeseed oil, ultra performance liquid chromatography (UPLC), solid-phase extraction(SPE)