食品科学 ›› 2013, Vol. 34 ›› Issue (21): 96-102.doi: 10.7506/spkx1002-6630-201321020

• 基础研究 • 上一篇    下一篇

5品种小麦胚芽胚乳蛋白质、赖氨酸和谷胱甘肽的比较研究

张艳贞,高 燕,张 静,陈 文*   

  1. 北京联合大学应用文理学院,北京 100191
  • 收稿日期:2013-06-26 修回日期:2013-10-12 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 陈文 E-mail:wlchenwen@buu.edu.cn
  • 基金资助:

    北京联合大学人才强校计划项目(BPHR2012C01);北京联合大学应用文理学院教学质量提高项目(21203546901)

Comparative Study of Protein, Lysine and Glutathione Extracted from Wheat Germ or Endosperm in Five Cultivars

ZHANG Yan-zhen,GAO Yan,ZHANG Jing,CHEN Wen*   

  1. College of Applied Arts and Science, Beijing Union University, Beijing 100191, China
  • Received:2013-06-26 Revised:2013-10-12 Online:2013-11-15 Published:2013-10-28
  • Contact: CHEN Wen E-mail:wlchenwen@buu.edu.cn

摘要:

目的:比较周麦16、Spelt7、中优9507、Spelt53、小偃54的小麦胚芽胚乳中蛋白质、赖氨酸和谷胱甘肽含量和性质差异。方法:蛋白质性质分析采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和聚丙烯酰胺等电聚焦电泳(IEF-PAGE)方法;蛋白质、赖氨酸和谷胱甘肽含量测定分别采用Folin-酚法、茚三酮法和DTNB法。结果:同一品种胚芽、胚乳间蛋白质亚基组成及数目有所不同;品种间胚芽、胚乳中蛋白质亚基分子质量和等电点分布范围相似;品种间胚芽蛋白质含量及谷胱甘肽含量普遍存在显著差异(P<0.05)或极显著差异(P<0.01);同一品种内胚芽蛋白质、赖氨酸和谷胱甘肽含量通常高于胚乳,且差异显著(P<0.05)或极显著(P<0.01)。结论:小麦胚芽蛋白质和谷胱甘肽普遍存在种质差异,且三者含量胚芽普遍高于胚乳。总体评价以中优9507品质最好。

关键词: 小麦胚芽, 胚乳, 蛋白质, 赖氨酸, 谷胱甘肽

Abstract:

Purpose: To compare the content and properties of protein, lysine and glutathione extracted from wheat germ or
endosperm from five cultivars (Zhoumai 16, spelt 7, Zhongyou 9507, spelt 53 and Xiaoyan 54). Methods: Protein properties were
characterized by SDS-PAGE and IEF-PAGE. The contents of protein, lysine and glutathione were determined using Folin-phenol
assay, ninhydrin reaction and DTNB colorimetry, respectively. Results: The composition and number of protein subunits of wheat
germ were different from those of endosperm in each cultivar. The molecular weights and isoelectric points of protein subunits had
similar distribution range between wheat germ and endosperm in five cultivars. The contents of wheat germ protein and glutathione
indicated significant differences among different cultivars (P < 0.05 or P < 0.01). Protein, lysine and glutathione contents of
wheat germ from the same cultivar were much higher than those of endosperm with significant difference (P < 0.05 or P < 0.01).
Conclusions: Inter-cultivar diversities of wheat germ protein and glutathione contents existed resulting from germplasm difference,
and the contents of protein, lysine and glutathione in wheat germ were commonly higher than in endosperm. As a whole, Zhongyou
9507 was the best in quality.

Key words: wheat germ, endosperm, protein, lysine, glutathione

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