食品科学 ›› 2014, Vol. 35 ›› Issue (1): 174-179.doi: 10.7506/spkx1002-6630-201401034

• 生物工程 • 上一篇    下一篇

粗壮脉纹胞菌复合多菌种发酵茶粕产纤维素酶的研究

刘沛毅,邓泽元,杨建远,李 静*   

  1. 南昌大学高等研究院,食品科学与技术国家重点实验室,江西 南昌 330047
  • 收稿日期:2013-07-01 修回日期:2013-11-20 出版日期:2014-01-15 发布日期:2014-01-22
  • 通讯作者: 李静 E-mail:jingjingbailu@yahoo.com.cn
  • 基金资助:

    食品科学与技术国家重点实验室基金项目(SKLF-QN-201105;SKLF-ZZA-201303);江西省自然科学基金项目(2010BN1301104)

Cellulase Production from Tea Seed (Camellia oleifera) Cake Using Neurospora crassa Mixed with Other Bacteria

LIU Pei-yi, DENG Ze-yuan, YANG Jian-yuan, LI Jing*   

  1. State Key Laboratory of Food Science and Technology, Institute for Advanced Studies, Nanchang University, Nanchang 330047, China
  • Received:2013-07-01 Revised:2013-11-20 Online:2014-01-15 Published:2014-01-22
  • Contact: LI Jing E-mail:jingjingbailu@yahoo.com.cn

摘要:

用粗壮脉纹胞菌分别复合东方伊莎酵母、里氏木霉、绿色木霉、乳酸杆菌固态发酵已去除茶皂素的茶粕,通过测定发酵产物中3种纤维素酶:外切葡聚糖酶(C1)、内切葡聚糖酶(Cx)、β-葡萄糖苷酶(β-G)及总酶滤纸酶(filter paper activity,FPA)的酶活力来探讨其分解粗纤维素的协同作用。粗壮脉纹胞菌和绿色木霉混合发酵产生的C1酶酶活力较粗壮脉纹胞菌单菌发酵提高了51.09%,粗壮脉纹胞菌和绿色木霉复合发酵较单菌发酵延长了其纤维素酶分泌的周期,96 h时FPA酶活力达到2.782 U/g;粗壮脉纹胞菌复合里氏木霉、绿色木霉混合发酵组在发酵10 d后对茶粕粗纤维的最终降解率分别达到了64.19%和61.59%;接种量对单菌和混合菌发酵产纤维素酶影响总体趋势是随着接种量增加酶活力提高,但粗壮脉纹胞菌单菌发酵纤维素酶酶活力在接种量超过9 mL/100 g后开始下降。表明粗壮脉纹胞菌复合里氏木霉、绿色木霉混合发酵降解纤维素具有协同作用。

关键词: 粗壮脉纹胞菌, 纤维素酶, 混合菌发酵, 固态发酵, 茶粕

Abstract:

The production of cellulaes from tea seed (Camellia oleifera) cake with a minimized content of residual tea
saponin of 0.917% as obtained by ultrasonic extraction method was studied in solid-state fermentation by mixed strains of
Neurospora crassa with Issatchenkia oriental, Trichoderma reesei, Trichoderma vivide or L. plantarum. The activities of
cellobiohydrolase 1 (C1), β-1,4-endoglucanase (Cx) and β-glucosidase (β-G) and filter paper activity (FPA) in fermentation
supernatants were determined to explore their synergistic action on enzymatic hydrolysis of cellulose. The enzyme activity of
C1 from Neurospora crassa combined with Trichoderma vivide was increased by 51.09% compared with that obtained from
single-strain fermentation by Neurospora crassa; meanwhile, the combination of both strains resulted in prolonged secretion
of cellulases, and the FPA activity reached 2.782 U/g after 96 h. The cellulose was degraded by 64.19% and 61.59% in tea
seed cake fermented for 10 days by Neurospora crassa mixed with Trichoderma reesei and Trichoderma vivide, respectively.
The overall tendency of the enzyme activities was an increase with increasing the inoculum size irrespective of fermentation
with Neurospora crassa alone or combined with other strains. However, the cellulase activities were declined at higher
inoculum sizes surpassing 9 mL/100 g of Neurospora crassa when inoculated alone. These results indicate that Neurospora
crassa is able to cooperate with Trichoderma reesei and Trichoderma vivide respectively to degrade cellulose.

Key words: Neurospora crassa, cellulases, mixed-bacteria fermentation, solid-state fermentation, tea seed

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