食品科学

• 基础研究 • 上一篇    下一篇

红发夫酵母中3R,3’R-虾青素的分离纯化和结构鉴定

孙伟红,林 洪,翟毓秀,冷凯良,邢丽红   

  1. 1.中国海洋大学食品科学与工程学院,山东 青岛 266003;2.中国水产科学研究院黄海水产研究所,山东 青岛 266071
  • 发布日期:2014-07-03

Separation, Purification and Identification of (3R,3’R)-trans-Astaxanthin from Phaffia rhodozyma

SUN Wei-hong, LIN Hong, ZHAI Yu-xiu, LENG Kai-liang, XING Li-hong   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
    2. Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  • Published:2014-07-03

摘要:

研究红发夫酵母中3R,3’R-虾青素的分离纯化工艺条件,并利用高效液相色谱串联大气压化学电离源质谱(high performance liquid chromatography-atmospheric pressure chemical ionization- mass spectrometry,HPLCAPCI-MS)、核磁共振(nuclear magnetic resonance,NMR)和高效液相色谱-紫外光谱(high performance liquidchromatography-ultra violet,HPLC-UV)对纯化得到的虾青素结构进行鉴定。结果表明:利用细胞玻璃研磨器进行研磨提取、硅胶柱层析分离和结晶提纯相结合的方法,能够有效减少反式虾青素的异构化,得到的虾青素纯度高于95%。采用高效液相色谱-质谱仪(high performance liquid chromatography-mass spectrometry,HPLC-MS)、超导核磁共振谱仪(1H和13C NMR)以及Chiralpak IC手性固定化纤维柱分析,显示制备的反式虾青素几乎都以3R,3’R结构组成。

关键词: 红发夫酵母, 3R, 3&rsquo, R-虾青素, 分离, 纯化, 结构鉴定

Abstract:

The separation and purification of (3R,3’R)-trans-astaxanthin from Phaffia rhodozyma were studied, and the
3R,3’R isomers were characterized by HPLC-APCI-MS, 1H and 13C NMR and HPLC-UV. The results showed that the
isomerization of trans-astaxanthin was effectively inhibited by combined use of glass tissue grinder for extraction, lowpressure
silica gel column chromatography for separation and crystallization of supersaturated astaxanthin in acetone for
purification, and the purity of all-trans-astaxanthin crystalline powder was verified to be above 95% by using HPLC. Then,
HPLC-APCI-MS, 1H and 13C NMR and chiralpak IC column based on cellulose tris(3,5-dichlorophenyl carbamate) polymer
confirmed that the trans-astaxanthin was composed almost exclusively of 3R,3’R isomer.

Key words: Phaffia rhodozyma, (3R,3’R)-trans-astaxanthin, separation, purification, identification