食品科学

• 生物工程 • 上一篇    下一篇

产褐藻胶裂解酶菌种的筛选、鉴定及发酵条件优化

陈 朋1,2,朱玥明2,韩文佳1,2,门 燕2,贾士儒1,孙媛霞2,*   

  1. 1.天津科技大学生物工程学院,天津 300457;2.中国科学院天津工业生物技术研究所,天津 300308
  • 出版日期:2015-08-15 发布日期:2015-08-17

Screening and Identification of a Bacterial Strain and Optimization of Medium Composition and Culture Conditions for the Production of Alginate Lyase

CHEN Peng1,2, ZHU Yueming2, HAN Wenjia1,2, MEN Yan2, JIA Shiru1, SUN Yuanxia2,*   

  1. 1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
    2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
  • Online:2015-08-15 Published:2015-08-17

摘要:

以褐藻酸钠为唯一碳源,经多次富集和驯化,从养殖场腐烂海带中筛选到一株高产褐藻胶裂解酶的菌株Alg07。依据菌体形态、生理生化特征和16S rRNA基因序列分析,归属于芽孢杆菌属(Bacillus),命名为Bacillusweihaiensis Alg07。通过单因素试验和正交试验,确定菌株Alg07的最佳发酵产酶培养基成分:褐藻酸钠9 g/L、蛋白胨1 g/L、酵母粉3 g/L、NaCl 5 g/L、MgSO4·7H2O 1 g/L、KCl 5 g/L、CaCl2 4 g/L;最佳发酵产酶条件为:250 mL三角瓶装液量40 mL、培养温度30 ℃、初始发酵pH 6.5、接种量0.5%、摇床转速180 r/min、培养时间24 h。在优化后的培养条件下,褐藻胶裂解酶活力由35 U/mL提高到563 U/mL。

关键词: 褐藻胶裂解酶, 筛选, 鉴定, 芽孢杆菌, 发酵条件优化

Abstract:

A highly efficient alginate-degrading microorganism was isolated from rotten seaweed through several cycles of
enrichment and domestication in liquid medium containing alginate as the sole carbon source. According to morphological,
physiological characteristics and 16S rRNA gene sequence analysis, the strain was identified as Bacillus weihaiensis Alg07.
Based on the results of single factor experiments and orthogonal array experiments, the optimal liquid fermentation medium
contained 9 g/L of alginate, 1 g/L of peptone, 3 g/L of yeast exact, 5 g/L of NaCl, 1 g/L of MgSO4·7H2O, 5 g/L of KCl and
4 g/L of CaCl2. The strain was cultured at 30 ℃, pH 6.5 and 180 r/min for 24 h in 40 mL of the medium using a 250-mL
flask, and the inoculum concentration was 0.5% (V/V). Under these optimal conditions, the activity of alginate lyase was
significantly increased from 35 U/mL to 563 U/mL, indicating a 16-fold increase compared with that before optimization.

Key words: alginate lyase, screening, identification, Bacillus, fermentation optimization

中图分类号: