食品科学

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DNA条形码技术在深圳鱼肉制品鉴定中的应用

王 敏1,刘 荭2,黄 海3,赵晓萌1,石 琼1,何舜平1,4,孙 颖1,*   

  1. 1.深圳华大基因研究院,广东 深圳 518083;2.深圳出入境检验检疫局动植物检验检疫技术中心,广东 深圳 518045;
    3.三亚市南繁科学技术研究院,海南 三亚 572000;4.中国科学院水生生物研究所,湖北 武汉 430072
  • 出版日期:2015-10-25 发布日期:2015-10-20
  • 通讯作者: 孙 颖
  • 基金资助:

    深圳市科技计划深港创新圈项目(SGLH20131010105856414);广东省省部产学研专项(2013B090800017)

Identifying Fish Products in Shenzhen through DNA Barcoding

WANG Min1, LIU Hong2, HUANG Hai3, ZHAO Xiaomeng1, SHI Qiong1, HE Shunping1,4, SUN Ying1,*   

  1. 1. BGI-Shenzhen, Shenzhen 518083, China; 2. Animal & Plant Inspection and Quarantine Technology Center,
    Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China;
    3. Sanya Science and Technology Academy for Crop Winter Multiplication, Sanya 572000, China;
    4. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Online:2015-10-25 Published:2015-10-20
  • Contact: SUN Ying

摘要:

以线粒体细胞色素氧化酶亚基Ⅰ(COⅠ )基因为目标基因,应用DNA条形码技术鉴别深圳批发市场和超市零售鱼肉制品的种类来源,判别其产品标签是否正确。本研究调查的77 份鱼肉制品均能扩增出特异性条带,28 份样品与产品标签标示不符,“错贴”率高达36.36%,其中所有标示“龙俐鱼”的商品都是低价的“巴丁鱼”(Pangasianodon hypophthalmus)。DNA条形码技术可用于鱼肉制品的来源物种鉴定。

关键词: DNA条形码, COⅠ 基因, 物种鉴定, 鱼肉制品

Abstract:

In this study, DNA barcoding was applied to identify the exact species of fish products from Shenzhen wholesale
and retail markets and to check if these products are correctly labeled. Using mitochondrial cytochrome C oxidase subunit
Ⅰ (COⅠ ) gene as the target, the sequences of COⅠ from different samples were analyzed. Our results showed that the
COⅠ gene sequences of all 77 samples examined were successfully amplified by PCR. However, up to 36.36%(28/77)
of them were inconsistent with their label descriptions, in which all the products labeled with “tongue fish” were actually the
low-cost Pangasianodon hypophthalmus. As a simple, rapid and efficient technology, DNA barcoding can be widely used for
species identification of fish products.

Key words: DNA barcoding, mitochondrial cytochrome C oxidase subunit Ⅰ (COⅠ) gene, species identif??ication, fish products

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