食品科学

• 基础研究 • 上一篇    下一篇

超高压对肌球蛋白-海藻酸钠-氯化钙混合凝胶特性的影响

史可夫,肖 雄,吴双双,李月双,陈从贵*   

  1. 合肥工业大学生物与食品工程学院,安徽 合肥 230009
  • 出版日期:2015-12-15 发布日期:2015-12-24

Effect of High Pressure Processing on Properties of Chicken Breast Myosin Gel Containing Sodium Alginate and Calcium Chloride

SHI Kefu, XIAO Xiong, WU Shuangshuang, LI Yueshuang, CHEN Conggui*   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

本实验以含有0.5%海藻酸钠(sodium alginate,SA)和0.2%氯化钙(CaCl2)的鸡肉肌球蛋白混合体系(M-SA-CaCl2)为研究对象,考察超高压处理(high pressure processing,HPP)(100~400 MPa)对混合体系凝胶特性(凝胶保水性(water-holding capacity,WHC)和凝胶强度)的影响。通过分析M-SA-CaCl2混合体系表面疏水性、活性巯基含量、流变特性及凝胶微结构的变化,探讨混合凝胶特性的变化机制。结果表明: 1)混合凝胶WHC随着压力的提高(100~400 MPa)而显著增加(P<0.05),而凝胶强度随之显著降低(P<0.05); 2)HPP能够增加M-SA-CaCl2混合凝胶体系内部的活性巯基含量,强化混合体系内部疏水作用,减弱SA和CaCl2对肌球蛋白凝胶的增强作用,降低肌球蛋白的热凝胶能力以及凝胶网络结构的蛋白质聚集程度,由此导致混合凝胶特性的变化。

关键词: 超高压, 肌球蛋白, 海藻酸钠, 氯化钙, 凝胶特性

Abstract:

High pressure processing (HPP) as a non-thermal processing technology has broad prospects in the meat
industry. The effects of high pressure levels (100 – 400 MPa) on the properties of chicken breast myosin gel containing
0.5% (m/m) sodium alginate (SA) and 0.2% (m/m) calcium chloride (CaCl2) (M-SA-CaCl2) were investigated. The surface
hydrophobicity, reactive sulfhydryl content, rheological properties and gel microstructure of M-SA-CaCl2 were analyzed in
order to understand the mechanism of the changes in the gel properties. The results showed that the water-holding capacity (WHC)
of M-SA-CaCl2 was significantly (P < 0.05) increased with elevating pressure while the gel strength was decreased. These changes
could result from the significant (P < 0.05) increase in the surface hydrophobicity and reactive sulfhydryl content, weakened
thermal gelation ability of M-SA-CaCl2, and reduced protein aggregation within mixed gel after HPP treatment.

Key words: high pressure processing, myosin, sodium alginate, calcium chloride, gel properties

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