食品科学

• 生物工程 • 上一篇    下一篇

延边泡菜品质评价以及耐盐酵母菌的筛选

乌日娜1,2,孟令帅1,王茜茜1,于美玲1,岳喜庆1,武俊瑞1,*   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.江南大学食品学院,食品科学与技术国家重点实验室,江苏 无锡 214122
  • 出版日期:2015-03-15 发布日期:2015-03-17

Quality Evaluation of and Salt-Tolerant Yeast Screening from Yanbian Kimchi

WU Rina1,2, MENG Lingshuai1, WANG Qianqian1, YU Meiling1, YUE Xiqing1, WU Junrui1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. State Key Laboratory of Food Science and Technology, College of Food Science, Jiangnan University, Wuxi 214122, China
  • Online:2015-03-15 Published:2015-03-17

摘要:

从延边11 个泡菜样品中分离出37 株酵母菌,根据其子囊孢子、假菌丝和细胞形态等生理生化特征,可将其分为爱尼拉酵母属、拿逊酵母属和毕赤氏酵母属等9 个属。再从中筛选出6 株耐盐酵母菌。对6 株耐盐酵母菌的26S rDNA D1/D2区域进行测序,通过序列分析和构建系统发育树,进行种属鉴定。结果表明:6 株耐盐酵母菌分别来自两个属。LB1-3和YB1-3属于酿酒酵母属,LB1-3被鉴定为Kazachstania turicensis;YB1-3被鉴定为Kazachstaniaexigua。LD1-3、TB1-3、YL1-2和TL1-2属于酵母属,被鉴定均为赛瓦酵母(Saccharomyces servazzii)。

关键词: 泡菜, 酵母菌, 26S rDNA, 系统发育树, 同源性分析

Abstract:

Totally 37 yeasts were isolated from 11 kimchi samples from Yanbian prefecture, Jilin province, and classified
taxonomically into Eeniella, Nadsonia, Pichia and other 6 genera according to their ascospore, pseudohyphae and cell morphology.
Out of these isolates, six with salt tolerance were selected and identified by 26S rDNA D1/D2 regional sequence and phylogenetic
tree analyses. The results showed that the six strains with salt tolerance were from two yeast species. Among these strains,
LB1-3 and YB1-3 belonged to Kazachstania, LB1-3 was identified as Kazachstania turicensis, YB1-3 was identified as
Kazachstania exigua, and LD1-3, TB1-3, YL1-2 and TL1-2 were identified as Saccharomyces servazzii.

Key words: kimchi, yeast, 26S rDNA, phylogenetic tree, homology analysis

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