食品科学

• 工艺技术 • 上一篇    下一篇

响应面法优化藻油中抗氧化剂的复配组合

吕军伟1,2,杨贤庆1,*,林婉玲1,李来好1,马海霞1,魏 涯1   

  1. 1.中国水产科学研究院南海水产研究所,国家水产品加工技术研发中心,农业部水产品加工重点实验室,
    广东 广州 510300;2.上海海洋大学食品学院,上海 201306
  • 出版日期:2015-03-25 发布日期:2015-03-17
  • 通讯作者: 杨贤庆
  • 基金资助:

    海洋经济创新发展区域示范专项(GD2012-A01);国家海洋局海洋公益性行业科研专项(201005020)

Optimization of Antioxidant Combinations for Algal Oil by Response Surface Methodology

LÜ Junwei1,2, YANG Xianqing1,*, LIN Wanling1, LI Laihao1, MA Haixia1, WEI Ya1   

  1. 1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, National Research and Development Center for Aquatic
    Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2015-03-25 Published:2015-03-17
  • Contact: YANG Xianqing

摘要:

为了提高藻油氧化稳定性,对添加在藻油中的抗氧化剂组合进行优化。利用Box-Behnken试验设计并结合响应面分析法,以迷迭香粉(≥30%鼠尾草酸)、VE、VC棕榈酸酯添加量为试验因素,以藻油过氧化值(peroxidevalue,POV)、硫代巴比妥酸(thiobarbituric acid,TBA)值及二十二碳六烯酸(docosahexaenoic acid,DHA)含量为响应值,并进行二次回归设计建立模型,得到的最佳抗氧化剂组合为:迷迭香粉0.37‰、VE 0.11‰、VC棕榈酸酯0.20‰。该优化条件下藻油经过12 d烘箱实验后测得POV为9.26 meq/kg,TBA为0.35 mg/kg,DHA含量为30.27%,其与模型预测值相吻合,表明所建立的回归模型是切实可行的。

关键词: 藻油, 抗氧化剂, 响应面法

Abstract:

The concentrations of rosemary powder (containing 30% or more carnosic acid), VE and VC added together to
algal oil for improved oxidative stability were optimized using Box-Behnken experimental design combined with response
surface analysis based on peroxide value (POV), thiobarbituric acid (TBA) value and docosahexaenoic acid (DHA) content.
A quadratic regression model was established. The optimal combination of the three antioxidants was 0.37‰ rosemary
spices, 0.11‰ VE, and 0.20‰ VC palmitate. The POV value was 9.26 meq/kg, TBA value 0.35 mg/kg and DHA content
30.27% under the optimized conditions after 12 day of accelerated oven-oxidation test. A good agreement was observed
between the predicted and actual values indicating that the established model in this study is feasible.

Key words: algal oil, antioxidants, response surface methodology

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