石榴叶多酚对急性酒精性肝损伤小鼠肾脏、心脏及免疫器官的抗氧化作用

doi:10.7506/spkx1002-6630-201601037

摘要/Abstract

摘要：

目的：研究石榴叶多酚（purified polyphenols from Punica granatum L. leaves，PPPL）对酒精所致急性肝损伤小鼠肾脏、心脏、脾脏、胸腺的保护作用。方法：将66 只雄性小鼠随机分为空白对照组、模型对照组、阳性对照组（联苯双酯150 mg/（kg·d）），PPPL低、中、高剂量组（100、200、400 mg/（kg·d）），连续灌胃30 d，第31天除空白对照组外其余各组给予50%乙醇（12 mL/kg），建立小鼠急性肝损伤模型。将小鼠脱臼处死后取肾脏、心脏、脾脏及胸腺，测定其超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力及丙二醛（malondialdehyde，MDA）、还原型谷胱甘肽（glutathione，GSH）含量。结果：与模型对照组相比，PPPL各剂量组均能提高小鼠各脏器的SOD、CAT、GSH-Px活性和GSH含量，降低MDA含量。结论：PPPL对酒精性肝损伤小鼠肾脏、心脏及免疫器官具有明显的抗氧化作用。

关键词: 石榴叶多酚, 酒精性肝损伤, 抗氧化作用, 心脏, 肾脏, 免疫器官

Abstract:

Objective: To investigate the protective effect of purified polyphenols from leaves of Punica granatum L. (PPPL)
on kidney, heart, spleen and thymus in a mouse model of acute alcohol-induced hepatic injury. Methods: Sixty-six male mice
were randomly divided into blank control group, alcoholic injury model group, positive control group (administered with
150 mg/(kg·d) bifendate by gavage) and PPPL low, medium, and high dose groups (100, 200 and 400 mg/(kg·d)). All mice
were administered for 30 days. On the next day, the mice from all groups except the blank control group were administered with
50% alcohol (12 mL/kg), and then all the mice were subjected to dislocation death to assay the activities of SOD, CAT and GSH-Px
and the contents of MDA and GSH in kidney, heart, spleen and thymus. Results: Compared with the alcoholic injury model
group, the activities of SOD, CAT and GSH-Px and the contents of GSH in kidney, heart, spleen and thymus revealed an obvious
increase in the PPPL treatment groups while the contents of MDA were obviously decreased. Conclusion: PPPL has pronounced
antioxidant effect on kidney, heart and immune organs in mice with alcoholic hepatic injury.

Key words: purified polyphenols from leaves of Punica granatum L. (PPPL), alcohol liver injury, antioxidantion, heart, kidney, immune organs

中图分类号:

TS201.4

王美菊，陶明煊，牛文颖，朱成，叶理跃，邢佳. 石榴叶多酚对急性酒精性肝损伤小鼠肾脏、心脏及免疫器官的抗氧化作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201601037.

WANG Meiju, TAO Mingxuan, NIU Wenying, ZHU Cheng, YE Liyue, XING Jia. Antioxidant Effects of Purified Polyphenols from Leaves of Punica granatum L. on Kidney, Heart and Immune Organs in Mice with Acute Alcoholic Liver Injury[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201601037.

[1]	梅邢，王广，田瑞，刘宇，夏兰欣，田成，李伟，程超. 5种不同纯度藻蓝蛋白的抗氧化特性[J]. 食品科学, 2021, 42(5): 17-23.
[2]	吴文英，李文娟，李露，尹术华，赵文辉，姚于飞. 白藜芦醇对多柔比星引起的心脏毒性保护作用研究进展[J]. 食品科学, 2021, 42(5): 271-277.
[3]	常志尚，刘颖，苏爱，王文成，徐宏伟，姜雨杉，梁惠. 海兔素对酒精性肝损伤大鼠肝脏保护作用及机制[J]. 食品科学, 2020, 41(7): 131-139.
[4]	于兰兰，刘伟，周雅琳，李雍，秦勇，李睿珺，许雅君. 小麦低聚肽对急性酒精中毒小鼠抗氧化功能的影响[J]. 食品科学, 2020, 41(7): 159-163.
[5]	彭新颜，刘媛，贺红军，张敏，邵凌健，曾玉龙，王晓雨. 乳清多肽对抑制反复冻融猪肉糜氧化和改善品质的影响[J]. 食品科学, 2020, 41(4): 7-14.
[6]	黄颖，谭书明，陈萍，陈小敏，宋长军. 蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用[J]. 食品科学, 2020, 41(21): 154-159.
[7]	曲航，高鑫，伊娟娟，王振宇. 食源性天然产物对酒精性肝损伤的防护作用研究进展[J]. 食品科学, 2020, 41(17): 283-290.
[8]	云少君, 褚东阳, 何兴帅, 张文芳, 冯翠萍. 原花青素对铁超载大鼠肾脏铁含量、氧化应激及Fas、Bax基因表达的影响[J]. 食品科学, 2020, 41(13): 106-111.
[9]	韩瑜玮，刘洋洋，陈伟. 蛹虫草多糖酸奶对酒精性肝损伤昆明种小鼠的协同保护作用[J]. 食品科学, 2020, 41(1): 209-214.
[10]	卓怡云，吕婧，刘颖，王子龙，刘曼，梁惠. 纳豆激酶对大鼠酒精性肝损伤的改善效果及免疫调节作用[J]. 食品科学, 2019, 40(7): 156-162.
[11]	陈树俊，张君梅，李佳益，郑婕，邢慧雅，吴梦月. 杂粮功能醋粉体内降血脂、辅助降血糖和抗氧化作用[J]. 食品科学, 2019, 40(13): 155-160.
[12]	丁荣荣，姜启兴，王斌，夏文水，杨方，许艳顺. 壳寡糖对小鼠急性酒精性肝损伤的保护作用[J]. 食品科学, 2019, 40(13): 161-165.
[13]	龙晓珊，胡腾根，邹宇晓，廖森泰，王思远，林光月，黎尔纳，刘凡，李倩. 发酵和加工对桑椹抗氧化和降血糖作用的影响[J]. 食品科学, 2019, 40(11): 116-123.
[14]	佴逸凡，冯莉，廖剑桥，马琦，伯继芳，李梅，徐怀德. 核桃对东莨菪碱诱导的小鼠记忆障碍的预防作用[J]. 食品科学, 2019, 40(11): 188-194.
[15]	王瑶，王晓娜，张雪辉，殷建忠，潘红梅，吴志霜，冯月梅，吴少雄，王松梅. 云南小粒咖啡类黑精的抗氧化及减肥功能[J]. 食品科学, 2019, 40(1): 183-189.

石榴叶多酚对急性酒精性肝损伤小鼠肾脏、心脏及免疫器官的抗氧化作用

Antioxidant Effects of Purified Polyphenols from Leaves of Punica granatum L. on Kidney, Heart and Immune Organs in Mice with Acute Alcoholic Liver Injury

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价