食品科学

• 专题论述 • 上一篇    

高通量测序在食品微生物生态学研究中的应用

米其利1,2,李雪梅1,管 莹1,高 茜1,桂永发1,朱洲海1,夭建华1,*   

  1. 1.云南中烟工业有限责任公司技术中心,云南 昆明 650106;2.云南大学 云南省微生物研究所,云南 昆明 650091
  • 出版日期:2016-12-15 发布日期:2016-12-21

Application of High-Throughput Sequencing in Food Microbial Ecology: A Review

MI Qili1,2, LI Xuemei1, GUAN Ying1, GAO Qian1, GUI Yongfa1, ZHU Zhouhai1, YAO Jianhua1,*   

  1. 1. Technology Center of China Tobacco Yunnan Industrial Co. Ltd., Kunming 650106, China;
    2. Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China
  • Online:2016-12-15 Published:2016-12-21

摘要: 随着分子生物学的发展,越来越多新的研究投入到对食品微生物的研究中。高通量测序作为一种新兴的分子生物学技术,具有数据产出通量高、分析全面、灵敏、快速等特点,被广泛用于食品微生物生态学的研究中。本文对高通量测序的操作流程及其在食品微生物生态学研究中的应用进行总结,评价该技术的优势和局限性,并对其在食品微生物研究中的应用前景进行展望,以期为食品微生物生态学的研究提供参考。

关键词: 食品微生物生态学, 高通量测序, 多样性, 微生物区系

Abstract: With the development of molecular biology, a growing number of new strategies have been used in studies in food
microbiology. High-throughput sequencing, a newly burgeoning culture-independent technique, is characterized by rapid,
sensitive and comprehensive analysis of large volume of data and has been recently introduced into food microbial ecology.
In this review, we summarize the process of high-throughput sequencing and its application in food microbial ecology. The
limitations and advantages of this technique are discussed, and future prospects for its application in food microbial ecology
are presented as well. The aim is to provide a reference for the development of food microbial ecology.

Key words: food microbial ecology, high-throughput sequencing, diversity, microflora

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