食品科学

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高效毛细管电泳用于检测黄花菜中4 种活性物质

郭芳芳,冯 锋*,白云峰,刘荔贞,陈泽忠,周静清,曹雨迪   

  1. 山西大同大学化学与环境工程学院,山西 大同 037009
  • 出版日期:2016-02-25 发布日期:2016-02-23
  • 通讯作者: 冯 锋
  • 基金资助:

    国家自然科学基金面上项目(21375083)

Determination of Four Active Compounds in Nightlily (Hemerocallis citrina) Flowers by High Performance Capillary Electrophoresis

GUO Fangfang, FENG Feng*, BAI Yunfeng, LIU Lizhen, CHEN Zezhong, ZHOU Jingqing, CAO Yudi   

  1. College of Chemical and Environmental Engineering, Shanxi Datong University, Datong 037009, China
  • Online:2016-02-25 Published:2016-02-23
  • Contact: FENG Feng

摘要:

使用高效毛细管电泳法,对黄花菜中活性化合物芦荟大黄素、山柰酚、绿原酸和槲皮素进行检测。在最佳电泳条件为10 mmol/L Na2B4O7-H3BO3缓冲溶液、pH 9.15、紫外检测波长224 nm、30 kV的外加电压条件下进样5 s,4 种物质在6 min内实现了分离,4 种物质的线性范围分别为0.02~0.21、0.01~0.90、0.01~0.99、0.01~0.90 mg/mL,线性相关系数在0.997 6~0.999 6之间,检出限分别为2.45×10-5、1.41×10-5、1.78×10-5、8.63×10-6 mg/mL(RSN=3),平均回收率在97.5%~101.2%之间,相对标准偏差不大于4.76%。实验结果表明该方法快速准确,适合用于黄花菜中活性物质的检测。

关键词: 高效毛细管电泳, 黄花菜, 槲皮素

Abstract:

An efficient high performance capillary electrophoresis (HPCE) method was developed for the determination
of aloe-emodin, kaempferol, chlorogenic and quercetin in nightlily flowers (Hemerocallis citrina). Sample injection was
carried out by hydrodynamic injection for 5 s at 30 kV. The running buffer was 10 mmol/L Na2B4O7-H3BO3 (pH 9.15), and the
detection wavelength was set at 224 nm. Four target compounds were separated in 6 min. Under these optimal conditions, the linear
ranges for the above four compounds were 0.02–0.21, 0.01–0.90, 0.01–0.99, and 0.01–0.90 mg/mL with correlation coefficients
between 0.997 6 and 0.999 6, and the limits of detection were 2.45 × 10-5, 1.41 × 10-5, 1.78 × 10-5 and 8.63 × 10-6 mg/mL
(RSN = 3), respectively. The average recoveries were 97.5% to 101.2%, with a relative standard deviation (RSD) less than
4.76%. This method is fast, accurate and suitable for the detection of these active compounds in H. citrine flowers.

Key words: high performance capillary electrophoresis (HPCE), Hemerocallis citrina, quercetin

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