食品科学

• 工艺技术 •    下一篇

响应面试验优化盐地碱蓬总黄酮乙醇萃取工艺及其抗氧化、降糖、降脂活性

公衍玲,刘 菲,金 宏   

  1. 青岛科技大学化工学院药学系,山东 青岛 266042
  • 出版日期:2016-04-25 发布日期:2016-04-13

Optimization of Ethanol Extraction of Total Flavonoids from Suaeda salsa and Its Antioxidant, Hypoglycemic and Hypolipidemic Activities

GONG Yanling, LIU Fei, JIN Hong   

  1. Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
  • Online:2016-04-25 Published:2016-04-13

摘要:

盐地碱蓬系藜科碱蓬属1 a生草本植物,既可作药物也可作为食物被人们广泛接受。本研究优化了从碱蓬中提取总黄酮的工艺及条件,并研究了其体外抗氧化、降血糖、降血脂活性。通过乙醇回流提取法进行总黄酮提取,使用单因素试验结合中心组合设计-响应面法确定了最优的工艺参数。并且将提取的总黄酮进行体外清除羟自由基和氧自由基、抑制α-淀粉酶和脂肪酶实验。总黄酮的乙醇提取工艺优化条件为液固比31.40∶1、乙醇体积分数83.12%、回流温度90 ℃、回流时间128.40 min。预测和实际的总黄酮产量分别为0.896%和0.932%。体外实验表明,总黄酮可以清除羟自由基和氧自由基,并且可以抑制α-淀粉酶和脂肪酶的活性。本实验中最优提取条件容易实现并且有良好的重复性。结果表明,总黄酮作为一种潜在的抗氧化、降糖、降血脂的自然资源的成分,可为碱蓬的发展提供新的药理学依据。

关键词: 盐地碱蓬, 总黄酮, 抗氧化活性, 降糖活性, 降血脂活性

Abstract:

Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and foodplant. In the current study, the extraction of total flavonoids from S. salsa and the in vitro antioxidant, hypoglycemic andhypolipidemic activities of the extracts were investigated. The total flavonoids was extracted by refluxing with ethanol andthe selection and optimization of main experimental parameters were carried out using single factor experiments combinedwith central composite design-response surface methodology (CCD-RSM). The total flavonoids extracted from S. salsawere tested for hydroxyl and oxygen radical scavenging activity, and inhibitory activities against α-amylase and lipasein vitro. Results revealed that the optimal conditions for the extraction of total flavonoids were as following: liquid-tosolidratio, 31.40:1; ethanol concentration, 83.12%; reflux temperature, 90 ℃, and reflux time, 128.40 min. Under theseoptimized conditions, the predicted and actual values of total flavonoids yield were 0.896% and 0.932%, respectively.The in vitro antioxidant tests revealed that the total flavonoids could scavenge hydroxyl and oxygen radicals and showedinhibitory activity against α-amylase and lipase. The optimal extraction conditions obtained in this experiment are simple toperform and have good reproducibility. The results have demonstrated that the total flavonoids from S. salsa can be used asa potential source of natural antioxidant, hypoglycemic and hypolipidemic ingredients, which provides new pharmacologicalbasis for the development of S. salsa.

Key words: Suaeda salsa, total flavonoids, antioxidant activity, hypoglycemic activity, hypolipidemic activity

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