食品科学

• 基础研究 • 上一篇    下一篇

广枣黄酮清除自由基能力及抗氧化性能的细胞模型法评价

杨云舒,姜子涛*,李 荣   

  1. 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134
  • 出版日期:2016-05-15 发布日期:2016-05-18

Evaluation of Free Radical Scavenging Ability and Antioxidant Activity of Flavonoids from Choerospondias axillaris Fruits Using Cell Model  

YANG Yunshu, JIANG Zitao*, LI Rong   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Online:2016-05-15 Published:2016-05-18

摘要:

利用制备色谱将广枣黄酮分离成F1和F2两个组分。分别测定广枣黄酮、F1和F2的总抗氧化能力、清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基(•OH)以及超氧阴离子自由基(O2-•)的能力,并与常见的抗氧化剂VC进行对比。结果表明:广枣黄酮具有良好的抗氧化效果,总黄酮的抗氧化能力介于F1和F2之间;F1对DPPH自由基的清除能力以及F1和F2对•OH的清除能力均高于VC,而在总抗氧化能力以及清除O2-•方面F1和F2的效果均弱于VC。另外,细胞模型法评价结果显示,广枣黄酮可清除小鼠巨噬细胞RAW264.7内的活性氧,表现出明显的抗氧化活性。

关键词: 广枣黄酮, 总抗氧化能力, 自由基清除活性, 细胞抗氧化活性, 小鼠巨噬细胞RAW264.7

Abstract:

Flavonoids from the dried fruits of Choerospondias axillaris were separated using preparative chromatography
into two fractions (F1 and F2). The total antioxidant capacity, and DPPH radical, hydroxyl radical and superoxide anion
radical scavenging activities of F1 and F2 were evaluated using ascorbic acid (VC) as the reference. The results demonstrated
that total flavonoids from C. axillaris had obvious antioxidant capacity, which was between F1 and F2. The DPPH radical
scavenging activity of F1 and the hydroxyl radical scavenging activities of F1 and F2 were higher than those of VC, but total
antioxidant capacity and superoxide anion radical scavenging activity of F1 and F2 were lower than those of VC. In addition,
as evaluated using cell model, the flavonoids from C. axillaris showed significant antioxidant activity in mouse macrophages
(RAW264.7).

Key words: flavonoids from Choerospondias axillaris fruits, total antioxidant capacity, free radical scavenging activity, cellular antioxidant activity, mouse macrophages RAW264.7

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